Linked Life Data

7607692

Source:http://linkedlifedata.com/resource/pubmed/id/7607692

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-8-15
pubmed:abstractText
The V79 hamster cell line irs1 is a repair-deficient mutant hypersensitive to radiation and DNA-reactive chemical agents. Somatic cell hybrids were formed by fusing irs1 cells with human lymphocytes and selecting for complementation in medium containing concentrations of mitomycin C (MMC) that are toxic to irs1. Thirty-eight MMC-resistant hybrids showed extensive segregation of human chromosomes, with 35 of them retaining human chromosome 7, as indicated by molecular marker and cytogenetic analyses. Inter-Alu-PCR products from the DNA of hybrids, when used as fluorescence in situ hybridization probe onto normal human metaphases, indicated that one resistant hybrid was monochromosomal for chromosome 7 and that the three resistant hybrids shown to be negative for chromosome 7 markers have retained portions of chromosome 7, with region 7q36 being the smallest common region. MMC-sensitive subclones of a resistant hybrid lost human chromosome 7. Therefore, the gene complementing the repair defect, XRCC2 (X-ray repair cross complementing), is assigned to human chromosome 7q36.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0888-7543
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
26
pubmed:geneSymbol
SRCC2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
619-22
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Assignment of the XRCC2 human DNA repair gene to chromosome 7q36 by complementation analysis.
pubmed:affiliation
Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, California 94551-0808, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't