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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1995-8-17
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pubmed:abstractText |
Endocytosis of the fluid phase marker, horse radish peroxidase, into baby hamster kidney cells is inhibited by treatment of cells with the fungal metabolite wortmannin. The IC50 of approximately 5 nM is consistent with the well-described action of wortmannin upon phosphatidylinositol (PI) 3-kinase. Analysis of the kinetics of uptake indicates a > 50% decrease in the initial rate of marker internalisation, a concomitant decrease in the volume of the early endosome and an increased efficiency of recycling of that marker which is internalised. As PI 3-kinase binds to activated growth factor receptors our data suggest that receptor activation can be coupled to receptor internalisation (down regulation) by localising PI 3-kinase stimulation of endocytosis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
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pubmed:month |
Jul
|
|
pubmed:issn |
0014-5793
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
367
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
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pubmed:pagination |
272-4
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|
pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
|
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pubmed:year |
1995
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pubmed:articleTitle |
Phosphatidylinositol 3-kinase regulation of fluid phase endocytosis.
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pubmed:affiliation |
Physiological Laboratory, University of Liverpool, UK.
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|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|
