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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
1995-8-10
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pubmed:abstractText |
This report describes an efficient strategy for determining the functions of sequenced genes in microorganisms. A large population of cells is subjected to insertional mutagenesis. The mutagenized population is then divided into representative samples, each of which is subjected to a different selection. DNA is prepared from each sample population after the selection. The polymerase chain reaction is then used to determine retrospectively whether insertions into a particular sequence affected the outcome of any selection. The method is efficient because the insertional mutagenesis and each selection need only to be performed once to enable the functions of thousands of genes to be investigated, rather than once for each gene. We tested this "genetic footprinting" strategy using the model organism Saccharomyces cerevisiae.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7604017-1317268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7604017-1334021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7604017-1664403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7604017-1714384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7604017-2199320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7604017-2555252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7604017-2558956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7604017-2569741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7604017-2828155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7604017-2982495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7604017-372045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7604017-375221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7604017-6212932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7604017-6320003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7604017-8388781
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
92
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pubmed:geneSymbol |
ILV2,
LEU2,
LYS2,
TRP1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6479-83
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7604017-Amino Acids,
pubmed-meshheading:7604017-Base Sequence,
pubmed-meshheading:7604017-DNA,
pubmed-meshheading:7604017-DNA, Fungal,
pubmed-meshheading:7604017-DNA Primers,
pubmed-meshheading:7604017-Genes,
pubmed-meshheading:7604017-Genes, Fungal,
pubmed-meshheading:7604017-Genetic Techniques,
pubmed-meshheading:7604017-Molecular Sequence Data,
pubmed-meshheading:7604017-Mutagenesis, Insertional,
pubmed-meshheading:7604017-Polymerase Chain Reaction,
pubmed-meshheading:7604017-Retroelements,
pubmed-meshheading:7604017-Saccharomyces cerevisiae
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pubmed:year |
1995
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pubmed:articleTitle |
Genetic footprinting: a genomic strategy for determining a gene's function given its sequence.
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pubmed:affiliation |
Department of Genetics, Stanford University, CA, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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