Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
1995-8-10
pubmed:abstractText
We have investigated the mitogenic effect of three mutant forms of human insulin on insulin-producing beta cells of the developing pancreas. We examined transgenic embryonic and adult mice expressing (i) human [AspB10]-proinsulin/insulin ([AspB10]ProIN/IN), produced by replacement of histidine by aspartic acid at position 10 of the B chain and characterized by an increased affinity for the insulin receptor; (ii) human [LeuA3]insulin, produced by the substitution of leucine for valine in position 3 of the A chain, which exhibits decreased receptor binding affinity; and (iii) human [LeuA3, AspB10]insulin "double" mutation. During development, beta cells of AspB10 embryos were twice as abundant and had a 3 times higher rate of proliferation compared with beta cells of littermate controls. The mitogenic effect of [AspB10]ProIN/IN was specific for embryonic beta cells because the rate of proliferation of beta cells of adults and of glucagon (alpha) cells and adrenal chromaffin cells of embryos was similar in AspB10 mice and controls. In contrast to AspB10 embryos, the number of beta cells in the LeuA3 and "double" mutant lines was similar to the number in controls. These findings indicate that the [AspB10]ProIN/IN analog increased the rate of fetal beta-cell proliferation. The mechanism or mechanisms that mediate this mitogenic effect remain to be determined.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-1713869, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-1769434, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-1963405, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-2172801, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-2387246, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-2551043, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-2657740, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-2674692, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-2829358, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-3057496, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-3197646, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-3282675, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-3301864, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-3306677, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-3360216, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-3449366, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-3511099, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-3537011, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-3771541, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-3902543, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-6096045, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-6376081, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-6995478, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-7516864, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-7867492, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-7903631, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-8402901, http://linkedlifedata.com/resource/pubmed/commentcorrection/7603977-8402902
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6239-43
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
A transgene coding for a human insulin analog has a mitogenic effect on murine embryonic beta cells.
pubmed:affiliation
Department of Anatomy and Cell Biology, State University of New York, Brooklyn 11203, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't