Linked Life Data

7602101

Source:http://linkedlifedata.com/resource/pubmed/id/7602101

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-8-7
pubmed:abstractText
Previously, we reported that the expression of hsp65 within and on host macrophages correlates closely with protection against infection with Toxoplasma gondii in mice. Herein, we propose that gamma delta T cells play a crucial role in the induction of hsp65 and also in the protective immune response to T. gondii. Intraperitoneal inoculation with this protozoan resulted in hsp65 being expressed on and in host peritoneal macrophages and resulted in an increase of T cells bearing the gamma delta receptor with Thy-1+ and Thy-1- phenotypes in the peritoneal cavity and spleen. When mice were depleted of gamma delta T cells by the administration of a mAb, hsp65 expression was markedly decreased. In contrast, the expression of this protein was rather enhanced and gamma delta T cells were prominently expanded in mice depleted of alpha beta T cells. The protection in mice treated with the mAb paralleled the magnitude of hsp65 expression. Mice depleted of gamma delta T cells died most frequently in the early stages of infection, whereas most of those depleted of alpha beta T cells survived the early stages of lethal infection with T. gondii. However, the latter group of mice did not definitely control the T. gondii infection in its late stages. IFN-gamma was not essential for either the expression of hsp65 or the resistance induced by gamma delta T cells, as demonstrated in mice treated with mAb to murine IFN-gamma. These findings indicated that gamma delta T cells having both the Thy-1+ and Thy-1- phenotypes contribute to hsp65 expression within and on macrophages in an IFN-gamma-independent manner. This, in turn, plays a role in the development of protective immunity during the early stage of this parasitic infection.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
155
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
244-51
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7602101-Animals, pubmed-meshheading:7602101-Bacterial Proteins, pubmed-meshheading:7602101-Blotting, Western, pubmed-meshheading:7602101-Chaperonin 60, pubmed-meshheading:7602101-Chaperonins, pubmed-meshheading:7602101-Female, pubmed-meshheading:7602101-Flow Cytometry, pubmed-meshheading:7602101-Immunization, Passive, pubmed-meshheading:7602101-Immunohistochemistry, pubmed-meshheading:7602101-Interferon-gamma, pubmed-meshheading:7602101-Lymphocyte Depletion, pubmed-meshheading:7602101-Mice, pubmed-meshheading:7602101-Mice, Inbred BALB C, pubmed-meshheading:7602101-Microscopy, Immunoelectron, pubmed-meshheading:7602101-Receptors, Antigen, T-Cell, gamma-delta, pubmed-meshheading:7602101-T-Lymphocytes, pubmed-meshheading:7602101-Toxoplasma, pubmed-meshheading:7602101-Toxoplasmosis, Animal
pubmed:year
1995
pubmed:articleTitle
Gamma delta T cells play an important role in hsp65 expression and in acquiring protective immune responses against infection with Toxoplasma gondii.
pubmed:affiliation
Department of Parasitology and Immunology, University of Tokushima, School of Medicine, Japan.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't