Linked Life Data

7601410

Source:http://linkedlifedata.com/resource/pubmed/id/7601410

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-8-9
pubmed:abstractText
A problem traditionally encountered with primary hepatocyte cultures is their rapid dedifferentiation, which is reflected not only in decreased liver-specific functions, but also in dedifferentiated morphology: the cells flatten, depolarize, and lose many of the surface characteristics of normal hepatocytes in vivo. However, culture conditions that maintain primary rat hepatocytes in a healthy and highly differentiated state were recently developed: the hepatocytes are cultured in Chee's Medium supplemented with dexamethasone and dimethyl sulfoxide (DMSO) on collagen-coated Permanox dishes. In addition to retaining labile hepatocyte-specific functions (e.g., P450 activity and albumin synthesis), these hepatocytes also have a differentiated morphology. They have numerous microvilli and are cuboidal and cluster into cords reminiscent of hepatic trabeculae. Their subcellular organelles have normal morphology, and specialized junctions and bile canaliculi form within the membranes of adjacent cells. Actin fibers cluster at these canalicular surfaces. These hepatocytes also synthesize blood clotting factors, which aggregate into fibrin meshworks between cells. Taken together, these morphological data suggest that these hepatocytes are polarized and generally have an appearance very similar to parenchymal cells in the liver, and that the same culture conditions that promote retention of liver-specific functions are also critical to the maintenance of physiological morphology. In contrast to other hepatocyte cultures, this differentiated morphology, including the polarized nature of the cells, is established without the use of serum or flexible or complex extracellular matrices and shows a close link between cellular architecture and tissue-specific function.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0270-9139
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
175-87
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
A morphological study of differentiated hepatocytes in vitro.
pubmed:affiliation
W. R. Grace & Co.-Conn., Research Division, Columbia, MD 21044, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't