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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
|
pubmed:dateCreated |
1995-8-10
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pubmed:abstractText |
We have developed a totally automated fluorescence polarization immunoassay for homocyst(e)ine with no pretreatment or chromatographic steps. Comparison with four well-established chromatographic methods yielded r values ranging from 0.980 to 0.997 and slopes from 1.030 to 1.493. Inter- and intraassay CVs ranged from 0.0% to 8.0% and from 0.0% to 6.4%, respectively. Imprecision (CV) of measuring six plasma samples on three instruments ranged from 6.3% to 10.2%. The assay was linear for plasma samples diluted with buffer from 0 to 8-fold. Mean recovery of homocysteine added to two plasma samples was 97.1% and 99.9%. The assay exhibited almost no cross-reactivity towards cysteine and methionine, and a batch of 20 samples can be processed in 60 min.
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pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jul
|
pubmed:issn |
0009-9147
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
41
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
991-4
|
pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7600701-Autoanalysis,
pubmed-meshheading:7600701-Calibration,
pubmed-meshheading:7600701-Cardiovascular Diseases,
pubmed-meshheading:7600701-Fluorescence Polarization Immunoassay,
pubmed-meshheading:7600701-Homocysteine,
pubmed-meshheading:7600701-Humans,
pubmed-meshheading:7600701-Risk Factors,
pubmed-meshheading:7600701-Sensitivity and Specificity
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pubmed:year |
1995
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pubmed:articleTitle |
Rapid, fully automated measurement of plasma homocyst(e)ine with the Abbott IMx analyzer.
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pubmed:affiliation |
Abbott Diagnostics Division, Abbott Laboratories, Abbott Park, IL 60064, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study
|