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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-8-7
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pubmed:abstractText |
The two major promoters for transcription of the human mitochondrial genome are located near each other in the displacement-loop region of the molecule. Previous work has localized these promoters to regions of < 100 nucleotides each; the DNA sequence at the transcription start site is stringently required, as is the region from -10 to -40 base pairs upstream of each respective start site. Each upstream site is recognized and bound by human mitochondrial transcription factor A (h-mtTFA), an event previously shown to be important for transcriptional activation. We report here results using recombinant h-mtTFA that demonstrate the dependence of transcription initiation of h-mtTFA. In addition, altering the distance between the h-mtTFA binding site and the transcription start site greatly impairs transcription initiation efficiency. The decrease in transcription initiation efficiency was shown to be a consequence of altering the position of h-mtTFA binding as opposed to the strength of h-mtTFA binding, as judged by DNA footprinting ability. Analysis of a chimeric yeast-human promoter revealed that the yeast mtTFA homologue cannot substitute for the human protein, even when bound at an appropriate position upstream of the human transcription start site.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Mitochondrial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Directed RNA Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease I,
http://linkedlifedata.com/resource/pubmed/chemical/Mitochondrial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TFAM protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/mitochondrial transcription factor A
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
1271
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
127-34
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7599198-Base Composition,
pubmed-meshheading:7599198-Base Sequence,
pubmed-meshheading:7599198-Chimera,
pubmed-meshheading:7599198-Cloning, Molecular,
pubmed-meshheading:7599198-DNA, Mitochondrial,
pubmed-meshheading:7599198-DNA Replication,
pubmed-meshheading:7599198-DNA-Binding Proteins,
pubmed-meshheading:7599198-DNA-Directed RNA Polymerases,
pubmed-meshheading:7599198-Deoxyribonuclease I,
pubmed-meshheading:7599198-Escherichia coli,
pubmed-meshheading:7599198-Gene Expression,
pubmed-meshheading:7599198-Humans,
pubmed-meshheading:7599198-KB Cells,
pubmed-meshheading:7599198-Mitochondrial Proteins,
pubmed-meshheading:7599198-Molecular Sequence Data,
pubmed-meshheading:7599198-Mutagenesis, Insertional,
pubmed-meshheading:7599198-Nuclear Proteins,
pubmed-meshheading:7599198-Promoter Regions, Genetic,
pubmed-meshheading:7599198-Recombinant Proteins,
pubmed-meshheading:7599198-Saccharomyces cerevisiae,
pubmed-meshheading:7599198-Templates, Genetic,
pubmed-meshheading:7599198-Transcription, Genetic,
pubmed-meshheading:7599198-Transcription Factors
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pubmed:year |
1995
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pubmed:articleTitle |
Human mitochondrial transcription factor A and promoter spacing integrity are required for transcription initiation.
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pubmed:affiliation |
Department of Developmental Biology, Stanford University School of Medicine, CA 94305-5427, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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