Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1995-12-15
|
| pubmed:abstractText |
A synthetic peptide (25 amino acids) corresponding to a specific portion of the third intracytoplasmic loop of the rat serotonin 5-HT1B/1D beta receptor was coupled to keyhole limpet hemocyanin and injected monthly into rabbits. Anti-peptide antibodies were detected by enzyme-linked immunosorbent assay and characterized by immunoprecipitation of the 5-HT1B/1D beta receptor in CHAPS-solubilized extracts from rat striatal membranes. Up to 60% of solubilized striatal serotonin-O-carboxymethylglycyl[125I]iodotyrosinamide ([125I]GTI; a selective 5-HT1B/1D radioligand) binding sites were immunoprecipitated and subsequently pharmacologically identified as 5-HT1B receptors. The remaining 40% of [125I]GTI binding sites were shown to be 5-HT1D receptors. In addition, these antibodies were successfully used in immunofluorescence experiments to detect the 5-HT1B/1D beta, but not the 5-HT1D/1D alpha, receptor in transiently transfected LLC-PK1 cells. Immunoautoradiographic experiments performed with brain sections from the rat, mouse, and guinea pig showed that the substantia nigra and globus pallidus contained the highest densities of 5-HT1D beta receptor-like immunoreactivity. Comparison of the regional distribution of immunolabeling with that of the specific binding of [125I]GTI in the brain of these species further confirmed that the anti-peptide antibodies selectively recognized only the 5-HT1D beta component of [125I]GTI specific receptor binding sites.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Dipeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/serotonin-O-carboxymethyl-Gly-Tyr
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0022-3042
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
65
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2671-81
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7595565-Amino Acid Sequence,
pubmed-meshheading:7595565-Animals,
pubmed-meshheading:7595565-Autoradiography,
pubmed-meshheading:7595565-Binding Sites,
pubmed-meshheading:7595565-Corpus Striatum,
pubmed-meshheading:7595565-Dipeptides,
pubmed-meshheading:7595565-Guinea Pigs,
pubmed-meshheading:7595565-Immune Sera,
pubmed-meshheading:7595565-Immunologic Techniques,
pubmed-meshheading:7595565-Iodine Radioisotopes,
pubmed-meshheading:7595565-LLC-PK1 Cells,
pubmed-meshheading:7595565-Male,
pubmed-meshheading:7595565-Mice,
pubmed-meshheading:7595565-Molecular Sequence Data,
pubmed-meshheading:7595565-Rabbits,
pubmed-meshheading:7595565-Rats,
pubmed-meshheading:7595565-Rats, Sprague-Dawley,
pubmed-meshheading:7595565-Receptors, Serotonin,
pubmed-meshheading:7595565-Serotonin,
pubmed-meshheading:7595565-Swine,
pubmed-meshheading:7595565-Transfection
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Immunolabeling of central serotonin 5-HT1D beta receptors in the rat, mouse, and guinea pig with a specific anti-peptide antiserum.
|
| pubmed:affiliation |
INSERM U. 288, Neurobiologie Cellulaire et Fonctionnelle, Faculté de Médecine Pitié-Salpêtrière, Paris, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|