Linked Life Data

7594586

Source:http://linkedlifedata.com/resource/pubmed/id/7594586

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1995-12-14
pubmed:databankReference
pubmed:abstractText
We have isolated and characterized a canine class I MHC (dog leukocyte Ag, DLA) gene, DLA-79. The deduced protein sequence shares only 65% identity with a previously published canine class I cDNA, designated DLA-A, and exhibits 64% amino acid identity with the HLA-A, -B, -C consensus. The peptide-binding region of DLA-79 is unusual. Three of four highly conserved tyrosine residues (Tyr7, 59, 159, and 171), proposed to interact with the N terminus of peptide-Ag, are substituted. Additionally, the long alpha-helix lining the peptide-binding region in the alpha 1 domain contains one more amino acid residue than that observed in typical class I. Together, these features suggest that DLA-79 binds a distinct subset of peptides or other ligands. This gene has been expressed in a class I null human lymphoblastoid cell line, and the encoded heavy chain associated with beta 2-microglobulin and was transported to the cell surface. Ribonuclease protection analysis detected low levels of gene-specific mRNA in a broad variety of dog tissues. The highest levels were found in skeletal muscle, a tissue expressing relatively low levels of classical class I Ag. These data suggest that DLA-79 is functional and plays a specialized role in the immune response. Nucleotide sequence analysis of second exon sequences (encoding the alpha 1 domain) identified only two alleles in five dogs of different breeds; a third variant was found in a coyote. The divergent structure, relatively low mRNA expression, and limited polymorphism of this gene suggest that DLA-79 is not a classical or class Ia gene, but rather, an analogue of the MHC class Ib genes of humans and rodents.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
155
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4278-85
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Structure and expression of a divergent canine class I gene.
pubmed:affiliation
Transplantation Biology Program, Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't