rdf:type |
|
lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0013018,
umls-concept:C0026565,
umls-concept:C0039194,
umls-concept:C0086597,
umls-concept:C0205296,
umls-concept:C0221102,
umls-concept:C0332120,
umls-concept:C0392756,
umls-concept:C0439097,
umls-concept:C0591833,
umls-concept:C0596402,
umls-concept:C0856825,
umls-concept:C1167395,
umls-concept:C1527169,
umls-concept:C1547348,
umls-concept:C1552644,
umls-concept:C1705241,
umls-concept:C1823153,
umls-concept:C2349976
|
pubmed:issue |
9
|
pubmed:dateCreated |
1995-12-14
|
pubmed:abstractText |
NK-like cytotoxicity in F1-hybrid mice with acute GVH disease is mediated by donor-derived CD3+/CD4-/CD8- cells that can lyse both NK-sensitive YAC-1 target cells as well as NK-resistant targets such as BW1100 and P815. Our objective was to determine whether this activity is mediated by gamma delta TCR+ cells. We showed that NK-like cytotoxic activity in the spleen and lymph nodes of mice with acute GVH disease could be depleted by indirect complement-mediated lysis using an Ab against gamma delta TCR. When purified NK1.1+ spleen cells that had been positively selected on a magnetic cell separator were used as effector cells, we found that NK-like cytotoxicity was mediated only by gamma delta TCR+ cells, suggesting that cells with NK-like activity are gamma delta TCR+/NK1.1+. We showed by flow cytometry experiments that coexpression of NK1.1 and TCR-gamma delta occurred on a large proportion of large granular lymphocytes in the spleens of GVH mice, but was not detectable in normal control mice. In GVH mice, fewer than 10% of small agranular NK1.1+ lymphocytes coexpressed NK1.1+ and gamma delta TCR+. On the basis of this hypothesis, we postulate that graft-derived large granular lymphocytes develop the NK1.1+/gamma delta TCR+ phenotype during the reaction, and that these cells play a role in the pathogenesis of acute GVH disease. We performed experiments to determine whether depletion of gamma delta T cells from donor mice affected the outcome of lethal GVH disease and found that there was a significant reduction in mortality.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Ly,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Klrb1c protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/NK Cell Lectin-Like Receptor...,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1767
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
155
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4189-98
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7594574-Acute Disease,
pubmed-meshheading:7594574-Animals,
pubmed-meshheading:7594574-Antigens,
pubmed-meshheading:7594574-Antigens, Ly,
pubmed-meshheading:7594574-Antigens, Surface,
pubmed-meshheading:7594574-Cytotoxicity, Immunologic,
pubmed-meshheading:7594574-Female,
pubmed-meshheading:7594574-Flow Cytometry,
pubmed-meshheading:7594574-Graft vs Host Disease,
pubmed-meshheading:7594574-Immunophenotyping,
pubmed-meshheading:7594574-Killer Cells, Natural,
pubmed-meshheading:7594574-Lectins, C-Type,
pubmed-meshheading:7594574-Lymph Nodes,
pubmed-meshheading:7594574-Mice,
pubmed-meshheading:7594574-Mice, Inbred BALB C,
pubmed-meshheading:7594574-Mice, Inbred C3H,
pubmed-meshheading:7594574-Mice, Inbred C57BL,
pubmed-meshheading:7594574-Mice, Inbred DBA,
pubmed-meshheading:7594574-NK Cell Lectin-Like Receptor Subfamily B,
pubmed-meshheading:7594574-Proteins,
pubmed-meshheading:7594574-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:7594574-Spleen,
pubmed-meshheading:7594574-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:7594574-Weight Loss
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pubmed:year |
1995
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pubmed:articleTitle |
Gamma delta T cells in the pathobiology of murine acute graft-versus-host disease. Evidence that gamma delta T cells mediate natural killer-like cytotoxicity in the host and that elimination of these cells from donors significantly reduces mortality.
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pubmed:affiliation |
Department of Immunology, University of Manitoba, Winnipeg, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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