Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
1995-12-7
pubmed:abstractText
In the filamentous fungus Neurospora crassa, both the global-acting regulatory protein NIT2 and the pathway-specific regulatory protein NIT4 are required to turn on the expression of the nit-3 gene, which encodes nitrate reductase, the first enzyme in the nitrate assimilatory pathway. Three NIT2 binding sites and two NIT4 binding sites have been identified in the 1.3-kb nit-3 promoter region via mobility shift and footprinting experiments with NIT2-beta-galactosidase and NIT4-beta-Gactosidase fusion proteins. Quantitative mobility shift assays were used to examine the affinity of individual NIT2 binding sites for the native NIT2 protein present in N. crassa nuclear extracts. In vivo analysis of nit-3 promoter 5' deletion constructs and individual NIT2 and NIT4 binding-site deletions or mutations revealed that all of the NIT2 and NIT4 binding sites are required for the full level of expression of the nit-3 gene. A cluster of two NIT2 and two NIT4 binding sites located more than 1 kb upstream of the translational start site is required for nit-3 expression, and one NIT2 binding site and one NIT4 site, which are immediately adjacent to each other, are of particular functional importance. A significant NIT2-NIT4 protein-protein interaction might occur upon their binding to nearby sites.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-162536, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-1840634, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-2137552, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-2142156, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-2142530, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-2148685, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-2205530, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-2842795, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-2885741, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-6117784, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-7565720, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-7596294, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-7612627, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-7808401, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-796678, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-8096840, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-8257101, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-8286388, http://linkedlifedata.com/resource/pubmed/commentcorrection/7592372-8479443
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
177
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6093-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Binding affinity and functional significance of NIT2 and NIT4 binding sites in the promoter of the highly regulated nit-3 gene, which encodes nitrate reductase in Neurospora crassa.
pubmed:affiliation
Department of Biochemistry, Ohio State University, Columbus 43210, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.