Linked Life Data

7592171

Source:http://linkedlifedata.com/resource/pubmed/id/7592171

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-11-30
pubmed:abstractText
A study was performed to compare viable counts and bioluminescence for determining control related effective regrowth time (CERT) and postantibiotic effect (PAE) on Gram-negative bacteria after two hours of exposure to meropenem. There was a good correlation between bioluminescence and viable counts in determining the cell numbers in growing cultures of Escherichia coli. CERT was defined as the time required for the resumption of logarithmic growth and an increase of 1 log10 to occur over the pre-exposure inoculum in the test culture minus corresponding time for the control culture. PAE and CERT were studied on reference strains of Enterobacter cloacae, E. coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. At 4 x MIC of meropenem the CERTs of these four Gram-negative strains were 4.1, 4.9, 4.2, and 3.6 h, respectively, when assayed by bioluminescence. Corresponding CERTs using viable counts were 4.2, 5.0, 5.1 and 3.8 h, respectively. In contrast to this good agreement between the methods in assessing CERT, the corresponding PAEs were highly method dependent. At 4 x MIC of meropenem the PAEs on E. cloacae, E. coli, K. pneumoniae and P. aeruginosa were 3.9, 4.8, 4.7, and 3.5 h, respectively, when assayed by bioluminescene. However, the corresponding and simultaneously determined viable count PAEs were -0.4, 0.5, -0.1, and 0.7 h, respectively. The poor correlation between these methods in assessing the PAE is caused by greater initial decrease in viability compared with the less prominent initial change in cell density as measured by bioluminescence.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0305-7453
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
585-92
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Control-related effective regrowth time and post-antibiotic effect of meropenem on gram-negative bacteria studied by bioluminescence and viable counts.
pubmed:affiliation
Department of Infectious Diseases, University Hospital, Linköping, Sweden.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't