Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1995-12-1
pubmed:databankReference
pubmed:abstractText
Digestion of total nuclear DNA of the grasshopper Eyprepocnemis plorans with seven different restriction endonucleases (REs), and subsequent agarose gel electrophoresis, has shown the presence of highly repetitive DNA yielding the typical ladder-like banding pattern. The most clear pattern was produced by DraI, the monomer being some 180 bp. This repeat unit was subsequently cloned and sequenced. Bidirectional sequencing of five randomly chosen clones showed exactly the same nucleotides in all 180 positions. The possible explanations for such an extreme homogeneity of this tandem repeat are discussed in the light of current hypotheses on repetitive DNA function and molecular drive mechanisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0018-067X
pubmed:author
pubmed:issnType
Print
pubmed:volume
75 ( Pt 4)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
370-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Cloning and sequence analysis of an extremely homogeneous tandemly repeated DNA in the grasshopper Eyprepocnemis plorans.
pubmed:affiliation
Departamento de Genética, Facultad de Ciencias, Universidad de Granada, Spain.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.