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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1995-12-28
|
| pubmed:abstractText |
Over-production of gelatinase A (MMP-2) or under-production of its inhibitor (TIMP-2) may result in the matrix degradation crucial for metastasis, and early evaluation of their expression in primary tumor would offer important prognostic informations. RT-PCR amplicons of MMP-2 and TIMP-2 mRNA from tissue biopsies of 13 breast carcinomas and one fibrocystic mastopathy were quantitated. In comparison with their normal-tissue counterparts, their expression trends were not uniform: in some cases MMP-2 increased in the tumor without changes in TIMP-2, in others TIMP-2 expression also increased, although to a lesser extent than MMP-2; only in 2 cases was it slightly lower in the tumor tissue. Nevertheless, clearer insights were gained from the comparison of the ratio (R) between MMP-2tumor/normal and TIMP-2tumor/normal: as in the fibrocystic mastopathy, the R in carcinomas without lymph-node involvement (LN-) was usually lower than I in most cases. In contrast, in 5 out of 6 patients with lymph-node metastasis (LN+), the ratio ranged between 2 and 4. While the R magnitude was not related to the frequency of positive lymph nodes out of the total analyzed, nor to relapse status at follow-up (all relapse-free), the clear-cut difference between the LN- and LN+ groups was statistically significant. Results suggest that evaluation of MMP-2/TIMP-2 mRNA balance may constitute an early prognostic approach, which may also be more reliable concerning cancer aggressiveness as compared with the MMP-2 alone, and that boosting TIMP-2 expression may be a therapeutic strategy to prevent metastasis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Gelatinases,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloendopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Inhibitor of...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0020-7136
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
27
|
| pubmed:volume |
63
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
621-6
|
| pubmed:dateRevised |
2007-7-24
|
| pubmed:meshHeading |
pubmed-meshheading:7591276-Adult,
pubmed-meshheading:7591276-Aged,
pubmed-meshheading:7591276-Base Sequence,
pubmed-meshheading:7591276-Breast Neoplasms,
pubmed-meshheading:7591276-Disease Progression,
pubmed-meshheading:7591276-Female,
pubmed-meshheading:7591276-Gelatinases,
pubmed-meshheading:7591276-Gene Expression,
pubmed-meshheading:7591276-Humans,
pubmed-meshheading:7591276-Lymphatic Metastasis,
pubmed-meshheading:7591276-Matrix Metalloproteinase 2,
pubmed-meshheading:7591276-Metalloendopeptidases,
pubmed-meshheading:7591276-Middle Aged,
pubmed-meshheading:7591276-Molecular Sequence Data,
pubmed-meshheading:7591276-Neoplasm Staging,
pubmed-meshheading:7591276-Polymerase Chain Reaction,
pubmed-meshheading:7591276-Proteins,
pubmed-meshheading:7591276-RNA, Messenger,
pubmed-meshheading:7591276-Tissue Inhibitor of Metalloproteinase-2,
pubmed-meshheading:7591276-Transcription, Genetic
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Gelatinase A/TIMP-2 imbalance in lymph-node-positive breast carcinomas, as measured by RT-PCR.
|
| pubmed:affiliation |
Institute of Histology and General Embryology, Medical School, University of Padua, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|