Linked Life Data

7591133

Source:http://linkedlifedata.com/resource/pubmed/id/7591133

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1995-12-28
pubmed:abstractText
Type III group B streptococci (GBS) can be subdivided into three subtypes, RDP III-1, III-2, and III-3, on the basis of numerical analysis of HindIII restriction endonuclease digestion patterns (HindIII RDP) with their chromosomal DNAs. In the present study, the effect of C5a on opsonophagocytic killing of a representative strain from each RDP type was investigated by using a novel optical method for determining opsonophagocytic killing, and the effect of C5a-ase treatment of C5a on opsonophagocytic killing was also investigated. Pre-stimulation of polymorphonuclear leukocytes (PMNs) with C5a significantly increased opsonophagocytic killing of all three strains. The increase in killing was abolished by pretreating the C5a with GBS that express C5a-ase, a treatment that also destroyed the chemoattractant activity of the C5a. The kinetics of killing of the RDP III-2 strain differed from those of the other two strains. The survival of the RDP III-2 bacteria continued to decline over the entire 60-min incubation of the opsonophagocytic assay when PMNs were prestimulated with C5a or with C5a that had been inactivated with GBS C5a-ase (dC5a). In contrast, killing of the RDP III-1 and III-3 strains almost ceased after 20 or 60 min when PMNs were prestimulated with dC5a or C5a, respectively. A difference in bacterial killing between the III-2 strain and the III-1 and III-3 strains therefore became increasingly apparent with prolonged incubation time. The percentage of bacteria surviving in the extracellular fluid was approximately the same as the percentages of bacteria surviving in both intracellular and extracellular locations when PMNs were prestimulated with either C5a or dC5a. These data imply that the majority of bacterial killing occurred following phagocytosis and suggest that the enhanced killing of GBS following prestimulation of PMNs with C5a resulted from increased ingestion of the bacteria.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7591133-1398910, http://linkedlifedata.com/resource/pubmed/commentcorrection/7591133-1658325, http://linkedlifedata.com/resource/pubmed/commentcorrection/7591133-2551036, http://linkedlifedata.com/resource/pubmed/commentcorrection/7591133-2551146, http://linkedlifedata.com/resource/pubmed/commentcorrection/7591133-3053900, http://linkedlifedata.com/resource/pubmed/commentcorrection/7591133-65433, http://linkedlifedata.com/resource/pubmed/commentcorrection/7591133-6988524, http://linkedlifedata.com/resource/pubmed/commentcorrection/7591133-6989947, http://linkedlifedata.com/resource/pubmed/commentcorrection/7591133-7035562, http://linkedlifedata.com/resource/pubmed/commentcorrection/7591133-7107007, http://linkedlifedata.com/resource/pubmed/commentcorrection/7591133-790940, http://linkedlifedata.com/resource/pubmed/commentcorrection/7591133-8454345, http://linkedlifedata.com/resource/pubmed/commentcorrection/7591133-8455188, http://linkedlifedata.com/resource/pubmed/commentcorrection/7591133-8514389, http://linkedlifedata.com/resource/pubmed/commentcorrection/7591133-856869
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4764-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Role of C5a-ase in group B streptococcal resistance to opsonophagocytic killing.
pubmed:affiliation
Department of Microbiology, Joshi-Eiyoh University, Saitama, Japan.
pubmed:publicationType
Journal Article