Linked Life Data

7591017

Source:http://linkedlifedata.com/resource/pubmed/id/7591017

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-12-7
pubmed:abstractText
In the present study we describe the intracellular pathways for the transmission of growth signals by the potent vasoconstricting eicosanoids prostaglandin H2 and thromboxane A2 in smooth muscle cells from rat aorta. Carbocyclic thromboxane A2 and U46619 are stable thromboxane A2 mimetics acting at the common thromboxane A2/prostaglandin H2 receptor. Carbocyclic thromboxane A2 (10(-6) mol/L) induced an approximately 2.5-fold increase in [Ca2+]i above the basal value at 25 seconds. Maximal stimulation of the 42-kD mitogen-activated protein kinase isoform by both thromboxane A2 mimetics occurred at 5 minutes. Both thromboxane A2 mimetics at a concentration of 10(-6) mol/L induced the expression of c-fos and early growth response gene-1 (egr-1) mRNA, with a maximum at 30 minutes. Carbocyclic thromboxane A2 (10(-6) mol/L) induced a 3.3-fold increase in [3H]thymidine incorporation into cell DNA above the basal value and produced a 3.5-fold elevation of platelet-derived growth factor-BB-dependent [3H]thymidine incorporation into cell DNA. Similar effects of U46619 (10(-6) to 10(-5) mol/L) alone did in combination with platelet-derived growth factor-BB on cell DNA synthesis were obtained. The thromboxane A2/prostaglandin H2 receptor antagonist SQ29548 (10(-6) mol/L) completely suppressed the mitogenic effect of both thromboxane A2 mimetics (10(-6) mol/L). Pertussis toxin (10 to 100 ng/mL) did not influence the mitogenic effects of the thromboxane A2 mimetics. Carbocyclic thromboxane A2 (10(-6) mol/L) and platelet-derived growth factor-BB (20 ng/mL) per ser caused a 44% and 100% increase in cell number, respectively. In the presence of carbocyclic thromboxane A2 (10(-6) mol/L), platelet-derived growth factor-BB induced a 152% increase in cell number. Similar results were obtained with U46619 alone or in combination with platelet-derived growth factor-BB.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0194-911X
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
771-80
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:7591017-15-Hydroxy-11 alpha,9..., pubmed-meshheading:7591017-Animals, pubmed-meshheading:7591017-Aorta, pubmed-meshheading:7591017-Calcium, pubmed-meshheading:7591017-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:7591017-Cell Division, pubmed-meshheading:7591017-Cells, Cultured, pubmed-meshheading:7591017-DNA Replication, pubmed-meshheading:7591017-Muscle, Smooth, Vascular, pubmed-meshheading:7591017-Platelet-Derived Growth Factor, pubmed-meshheading:7591017-Prostaglandin Endoperoxides, Synthetic, pubmed-meshheading:7591017-Rats, pubmed-meshheading:7591017-Rats, Inbred WKY, pubmed-meshheading:7591017-Receptors, Thromboxane, pubmed-meshheading:7591017-Signal Transduction, pubmed-meshheading:7591017-Thromboxane A2, pubmed-meshheading:7591017-Vasoconstrictor Agents
pubmed:year
1995
pubmed:articleTitle
Thromboxane A2 and vascular smooth muscle cell proliferation.
pubmed:affiliation
Medizinische Universitäts-Poliklinik, Bonn, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't