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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
22
|
| pubmed:dateCreated |
1995-12-28
|
| pubmed:abstractText |
Transcription factors belonging to the NF-kappa B family are controlled by inhibitory I kappa B proteins, mainly I kappa B alpha and I kappa B beta. Apparently normal at birth, I kappa B alpha-/- mice exhibit severe runting, skin defects, and extensive granulopoiesis postnatally, typically dying by 8 days. Hematopoietic tissues from these mice display elevated levels of both nuclear NF-kappa B and mRNAs of some, but not all, genes thought to be regulated by NF-kappa B. NF-kappa B elevation results in these phenotypic abnormalities because mice lacking both I kappa B alpha and the p50 subunit of NF-kappa B show a dramatically delayed onset of abnormalities. In contrast to hematopoietic cells, I kappa B alpha-/- embryonic fibroblasts show minimal constitutive NF-kappa B, as well as normal signal-dependent NF-kappa B activation that is concomitant with I kappa B beta degradation. Our results indicate that I kappa b beta, but not I kappa B alpha, is required for the signal-dependent activation of NF-kappa B in fibroblasts. However, I kappa B alpha is required for the postinduction repression of NF-kappa B in fibroblasts. These results define distinct roles for the two forms of I kappa B and demonstrate the necessity for stringent control of NF-kappa B.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B p50 Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappaB inhibitor alpha
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0890-9369
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2736-46
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7590249-Animals,
pubmed-meshheading:7590249-Animals, Newborn,
pubmed-meshheading:7590249-Base Sequence,
pubmed-meshheading:7590249-DNA Primers,
pubmed-meshheading:7590249-DNA-Binding Proteins,
pubmed-meshheading:7590249-Female,
pubmed-meshheading:7590249-Fibroblasts,
pubmed-meshheading:7590249-Gene Expression Regulation,
pubmed-meshheading:7590249-Genes, Lethal,
pubmed-meshheading:7590249-Granulocytes,
pubmed-meshheading:7590249-Humans,
pubmed-meshheading:7590249-I-kappa B Proteins,
pubmed-meshheading:7590249-Mice,
pubmed-meshheading:7590249-Mice, Inbred C57BL,
pubmed-meshheading:7590249-Molecular Sequence Data,
pubmed-meshheading:7590249-NF-kappa B,
pubmed-meshheading:7590249-NF-kappa B p50 Subunit,
pubmed-meshheading:7590249-Phenotype,
pubmed-meshheading:7590249-Signal Transduction,
pubmed-meshheading:7590249-Up-Regulation
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Constitutive NF-kappa B activation, enhanced granulopoiesis, and neonatal lethality in I kappa B alpha-deficient mice.
|
| pubmed:affiliation |
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|