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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
21
|
| pubmed:dateCreated |
1995-12-26
|
| pubmed:abstractText |
The proteins and RNA regulatory elements that control tissue-specific pre-mRNA splicing in mammalian cells are mostly unknown. In this study, a set of proteins is identified that binds to a splicing regulatory element downstream of the neuron specific c-src N1 exon. This complex of proteins bound specifically to a short RNA containing the regulatory sequence in neuronal extracts that splice the N1 exon. It was not seen in non-neuronal cell extracts that fail to splice this exon. UV-cross-linking experiments identified a neuron-specific 75-kD protein and several nontissue-specific proteins, including the 53-kD heterogeneous nuclear ribonucleoprotein F (hnRNP F), as components of this complex. Although present in both extracts, hnRNP F binds tightly to the RNA only in the neuronal extracts. A mutation in the regulatory RNA sequence, that inhibits N1 splicing in vivo, abolished formation of the neuron-specific complex and the binding of the neuron-specific 75-kD protein. Competition experiments in the two extracts show that the binding of the neuronal protein complex to the src pre-mRNA is required to activate N1 exon splicing in vitro. Antibody inhibition experiments indicate that the hnRNP F protein is a functional part of this complex. The assembly of regulatory complexes from both constitutive and specific proteins is likely to be a general feature of tissue-specific splicing regulation.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Heterogeneous-Nuclear...,
http://linkedlifedata.com/resource/pubmed/chemical/Heterogeneous-Nuclear...,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0890-9369
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2659-71
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7590243-Animals,
pubmed-meshheading:7590243-Antibodies,
pubmed-meshheading:7590243-Base Sequence,
pubmed-meshheading:7590243-Exons,
pubmed-meshheading:7590243-Genes, src,
pubmed-meshheading:7590243-Heterogeneous-Nuclear Ribonucleoprotein Group F-H,
pubmed-meshheading:7590243-Heterogeneous-Nuclear Ribonucleoproteins,
pubmed-meshheading:7590243-Mice,
pubmed-meshheading:7590243-Molecular Sequence Data,
pubmed-meshheading:7590243-Nerve Tissue Proteins,
pubmed-meshheading:7590243-Neurons,
pubmed-meshheading:7590243-RNA, Messenger,
pubmed-meshheading:7590243-RNA Precursors,
pubmed-meshheading:7590243-RNA Splicing,
pubmed-meshheading:7590243-RNA-Binding Proteins,
pubmed-meshheading:7590243-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:7590243-Retinoblastoma,
pubmed-meshheading:7590243-Ribonucleoproteins,
pubmed-meshheading:7590243-Ultraviolet Rays
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
The generally expressed hnRNP F is involved in a neural-specific pre-mRNA splicing event.
|
| pubmed:affiliation |
Molecular Biology Institute, University of California at Los Angeles 90024-1662, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|