|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
21
|
|
pubmed:dateCreated |
1995-12-26
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
A protein has been identified that interacts specifically with both the Src homologous 3 (SH3) domain and carboxy-terminal sequences of the c-Abl tyrosine kinase. The cDNA encoding the Abl interactor protein (Abi-2), was isolated from a human lymphocyte library using the yeast two-hybrid system with the Abl SH3 domain as bait. Abi-2 binds to c-Abl in vitro and in vivo. Abi-2 is a novel protein that contains an SH3 domain and proline-rich sequences critical for binding to c-Abl. A basic region in the amino terminus of Abi-2 is homologous to the DNA-binding sequence of homeo-domain proteins. We show that Abi-2 is a substrate for the c-Abl tyrosine kinase. Expression of an Abi-2 mutant protein that lacks sequences required for binding to the Abl SH3 domain but retains binding to the Abl carboxyl terminus activates the transforming capacity of c-Abl. The properties of Abi-2 are consistent with a dual role as regulator and potential effector of the c-Abl protein and suggest that Abi-2 may function as a tumor suppressor in mammalian cells.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0890-9369
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
9
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2569-82
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:7590236-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:7590236-Amino Acid Sequence,
pubmed-meshheading:7590236-Binding Sites,
pubmed-meshheading:7590236-Cell Transformation, Neoplastic,
pubmed-meshheading:7590236-Cloning, Molecular,
pubmed-meshheading:7590236-Homeodomain Proteins,
pubmed-meshheading:7590236-Humans,
pubmed-meshheading:7590236-Lymphocytes,
pubmed-meshheading:7590236-Molecular Sequence Data,
pubmed-meshheading:7590236-Mutation,
pubmed-meshheading:7590236-Proline,
pubmed-meshheading:7590236-Protein-Tyrosine Kinases,
pubmed-meshheading:7590236-Proto-Oncogene Proteins c-abl,
pubmed-meshheading:7590236-RNA,
pubmed-meshheading:7590236-Sequence Homology, Amino Acid,
pubmed-meshheading:7590236-Substrate Specificity,
pubmed-meshheading:7590236-Tissue Distribution,
pubmed-meshheading:7590236-src Homology Domains
|
|
pubmed:year |
1995
|
|
pubmed:articleTitle |
Abi-2, a novel SH3-containing protein interacts with the c-Abl tyrosine kinase and modulates c-Abl transforming activity.
|
|
pubmed:affiliation |
Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27710, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
