Linked Life Data

7589826

Source:http://linkedlifedata.com/resource/pubmed/id/7589826

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1995-11-29
pubmed:abstractText
The presence of serum islet cell cytoplasmic antibodies (ICAs) is a standard autoimmune marker for insulin-dependent diabetes mellitus (IDDM). The antigenic molecule(s) responsible for ICA has not been identified, although antibodies to the 65-kDa isoform of glutamic acid decarboxylase (GAD65) do contribute. We tested 129 IDDM sera for antibodies to ICA512 (anti-ICA512), antibodies to GAD (anti-GAD), and ICAs; we tested for inhibition of ICAs with purified recombinant ICA512 and sheep brain GAD; and we tested for immunofluorescence reactivity on COS7 cells transfected with cDNA clones encoding ICA512 and GAD65. The results were that anti-ICA512 antibodies contribute to ICA reactivity and that these, in combination with anti-GAD antibodies, account for most ICA reactivity in IDDM. Anti-ICA512 antibodies were present at a frequency of 51% in 61 patients with early-onset IDDM (age of onset < or = 20 years) of short duration (< or = 1 month) but only in 9% of 68 patients with an onset age of > 20 years and/or a disease duration of > 1 month. The frequency of anti-GAD antibodies in these sera was similar irrespective of duration or age of onset. Anti-ICA512 and anti-GAD antibodies were demonstrable by indirect immunofluorescence on transfected COS7 cells, and ICA could be inhibited using either recombinant ICA512 or purified brain GAD. We conclude that anti-ICA512 and anti-GAD antibodies contribute to ICA reactivity and that anti-ICA512 antibodies account for the increased frequency of ICA reactivity in early-onset IDDM of short duration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0012-1797
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1290-5
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:7589826-Adolescent, pubmed-meshheading:7589826-Adult, pubmed-meshheading:7589826-Age of Onset, pubmed-meshheading:7589826-Animals, pubmed-meshheading:7589826-Autoantibodies, pubmed-meshheading:7589826-Autoantigens, pubmed-meshheading:7589826-Brain, pubmed-meshheading:7589826-Cell Line, pubmed-meshheading:7589826-Cercopithecus aethiops, pubmed-meshheading:7589826-Child, pubmed-meshheading:7589826-Child, Preschool, pubmed-meshheading:7589826-Diabetes Mellitus, Type 1, pubmed-meshheading:7589826-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:7589826-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:7589826-Glutamate Decarboxylase, pubmed-meshheading:7589826-Humans, pubmed-meshheading:7589826-Infant, pubmed-meshheading:7589826-Islets of Langerhans, pubmed-meshheading:7589826-Membrane Proteins, pubmed-meshheading:7589826-Protein Tyrosine Phosphatase, Non-Receptor Type 1, pubmed-meshheading:7589826-Protein Tyrosine Phosphatases, pubmed-meshheading:7589826-Receptor-Like Protein Tyrosine Phosphatases, Class 8, pubmed-meshheading:7589826-Recombinant Proteins, pubmed-meshheading:7589826-Reference Values, pubmed-meshheading:7589826-Sheep, pubmed-meshheading:7589826-Transfection
pubmed:year
1995
pubmed:articleTitle
Pancreatic islet cell cytoplasmic antibody in diabetes is represented by antibodies to islet cell antigen 512 and glutamic acid decarboxylase.
pubmed:affiliation
Centre for Molecular Biology and Medicine, Monash University, Victoria, Australia.
pubmed:publicationType
Journal Article, Comparative Study