Linked Life Data

7589799

Source:http://linkedlifedata.com/resource/pubmed/id/7589799

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-12-26
pubmed:databankReference
pubmed:abstractText
We have cloned chick homologues of the type-II activin receptor, which we have designated cActR-IIA and -IIB. Binding assays show that the two receptors are indistinguishable in their ability to bind activin-A, with comparable kds. Injection of mRNAs encoding these receptors into Xenopus embryos causes axial duplications. Expression of both receptors can first be detected in the primitive streak by in situ hybridization. This suggests that these genes may be activated in response to mesoderm induction. In agreement with this, we find that treatment of preprimitive streak chick embryos with activin-A leads to rapid induction of the expression of cActR-IIB. At later stages, cActR-IIA transcripts become localized mainly in the notochord and myotome and cActR-IIB in the dorsal neural tube, proximal-anterior part of the limb bud, sensory placodes, and specific regions of the fore- and midbrain. To test the response of early chick embryonic tissues to activin, we designed a new in vitro assay for differentiation. We find that explants of area opaca epiblast or posterior primitive streak from various stages can respond to activin treatment by differentiating into a variety of mesodermal cell types in a dose-dependent manner. These results suggest that the importance of activin-related signaling pathways is not confined to pregastrulation stages and that these receptors may be involved in mediating the effects of inducing signals during later stages of development of the mesoderm, limbs, and nervous system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:volume
172
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
192-205
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7589799-Activin Receptors, pubmed-meshheading:7589799-Activins, pubmed-meshheading:7589799-Amino Acid Sequence, pubmed-meshheading:7589799-Animals, pubmed-meshheading:7589799-Cell Differentiation, pubmed-meshheading:7589799-Cell Line, pubmed-meshheading:7589799-Cercopithecus aethiops, pubmed-meshheading:7589799-Chick Embryo, pubmed-meshheading:7589799-Cloning, Molecular, pubmed-meshheading:7589799-Dose-Response Relationship, Drug, pubmed-meshheading:7589799-Embryo, Nonmammalian, pubmed-meshheading:7589799-Gastrula, pubmed-meshheading:7589799-Gene Expression, pubmed-meshheading:7589799-Gene Library, pubmed-meshheading:7589799-Growth Substances, pubmed-meshheading:7589799-In Situ Hybridization, pubmed-meshheading:7589799-Inhibins, pubmed-meshheading:7589799-Mesoderm, pubmed-meshheading:7589799-Molecular Sequence Data, pubmed-meshheading:7589799-RNA, Messenger, pubmed-meshheading:7589799-Receptors, Growth Factor, pubmed-meshheading:7589799-Recombinant Proteins, pubmed-meshheading:7589799-Sequence Homology, Amino Acid, pubmed-meshheading:7589799-Transfection, pubmed-meshheading:7589799-Xenopus laevis
pubmed:year
1995
pubmed:articleTitle
Activin and its receptors during gastrulation and the later phases of mesoderm development in the chick embryo.
pubmed:affiliation
Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't