Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1995-12-7
|
| pubmed:abstractText |
HLA-G limited polymorphic gene maps to the human major histocompatibility complex (MHC) class I subregion and encodes the molecule which is the only MHC class I antigen expressed on cytotrophoblast cells at the maternal-fetal interface. In this tissue, HLA-G primary mRNA is differentially spliced. We have used a sensitive hot start reverse transcriptase-polymerase chain reaction (RT-PCR) technique to investigate the expression of HLA-G gene in first trimester trophoblasts and adult peripheral blood cells. PCR amplification with HLA-G primers specific of exon 3 has enabled us to demonstrate a novel alternatively spliced form of HLA-G mRNA present in fetal first trimester trophoblasts and lacking exon 4 (HLA-G4). Cloning the whole PCR product and hybridizing recombinant bacterial colonies with specific probes has permitted evaluation of HLA-G4 vs. full length mRNA frequency at approximately 1:200. Moreover, the presence of HLA-G transcripts was found at a very weak level in adult peripheral blood lymphocytes and equally in B- and T-cell populations. These results are relevant in the context of immune tolerance and in the potential use of HLA-G transcripts as a marker for RT-PCR detection of the fetal cells in maternal as a marker for RT-PCR detection of the fetal cells in maternal blood.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/HLA Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-G Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class I,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0015-5500
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
40
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
431-8
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7589701-Abortion, Induced,
pubmed-meshheading:7589701-Adult,
pubmed-meshheading:7589701-Alternative Splicing,
pubmed-meshheading:7589701-DNA Primers,
pubmed-meshheading:7589701-Embryo, Mammalian,
pubmed-meshheading:7589701-Exons,
pubmed-meshheading:7589701-Female,
pubmed-meshheading:7589701-Gene Expression,
pubmed-meshheading:7589701-HLA Antigens,
pubmed-meshheading:7589701-HLA-G Antigens,
pubmed-meshheading:7589701-Histocompatibility Antigens Class I,
pubmed-meshheading:7589701-Humans,
pubmed-meshheading:7589701-Lymphocytes,
pubmed-meshheading:7589701-Polymerase Chain Reaction,
pubmed-meshheading:7589701-Pregnancy,
pubmed-meshheading:7589701-Prenatal Diagnosis,
pubmed-meshheading:7589701-RNA, Messenger,
pubmed-meshheading:7589701-Transcription, Genetic,
pubmed-meshheading:7589701-Trophoblasts
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
HLA-G mRNA forms in human trophoblasts and peripheral blood lymphocytes: potential use in prenatal diagnosis.
|
| pubmed:affiliation |
CEA-DSV-DPTE, Laboratoire d'Immunoradiobiologie, Hôpital Saint-Louis, Centre Hayem, Paris, France.
|
| pubmed:publicationType |
Journal Article
|