Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-12-19
pubmed:abstractText
Utrophin is a large cytoskeletal protein which shows high homology to dystrophin. In contrast to the sarcolemmal distribution of dystrophin, utrophin accumulates at the postsynaptic membrane of the neuromuscular junction. Because of its localization within this compartment of muscle fibers, expression of utrophin may be significantly influenced by the presence of the motor nerve. We tested this hypothesis by denervating muscles of mdx mouse and monitoring levels of utrophin and its mRNA by immunofluorescence, immunoblotting and RT-PCR. A significant increase in the number of utrophin positive fibers was observed by immunofluorescence 3 to 21 days after sectioning of the sciatic nerve. Quantitative analyses of utrophin and its transcripts in hindlimb muscles denervated for two weeks showed only a moderate increase in the levels of both utrophin (approximately 2-fold) and its transcript (approximately 60 to 90%). The present data suggest that although utrophin is a component of the postsynaptic membrane, its neural regulation is distinct from that of the acetylcholine receptor.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
374
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
393-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7589578-Animals, pubmed-meshheading:7589578-Base Sequence, pubmed-meshheading:7589578-Cytoskeletal Proteins, pubmed-meshheading:7589578-Fluorescent Antibody Technique, pubmed-meshheading:7589578-Gene Expression, pubmed-meshheading:7589578-Immunoblotting, pubmed-meshheading:7589578-Membrane Proteins, pubmed-meshheading:7589578-Mice, pubmed-meshheading:7589578-Mice, Inbred mdx, pubmed-meshheading:7589578-Molecular Sequence Data, pubmed-meshheading:7589578-Muscle Denervation, pubmed-meshheading:7589578-Muscles, pubmed-meshheading:7589578-Muscular Dystrophies, pubmed-meshheading:7589578-Polymerase Chain Reaction, pubmed-meshheading:7589578-RNA, Messenger, pubmed-meshheading:7589578-RNA-Directed DNA Polymerase, pubmed-meshheading:7589578-Receptors, Cholinergic, pubmed-meshheading:7589578-Sciatic Nerve, pubmed-meshheading:7589578-Utrophin
pubmed:year
1995
pubmed:articleTitle
Expression of utrophin and its mRNA in denervated mdx mouse muscle.
pubmed:affiliation
Department of Physiology, Faculty of Medicine, University of Ottawa, Ont., Canada.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't