|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1995-12-12
|
|
pubmed:abstractText |
Two classes of high-affinity sites for [3H]cirazoline were characterized in rat brain and kidney membranes. In both tissues, the binding parameters for the high- and low-affinity sites are similar with Bmax values of approximately 50 fmol/mg protein, Kd approximately 0.6 nM and Bmax approximately 470 fmol/mg protein, Kd approximately 11 nM respectively. Inhibition studies of [3H]cirazoline binding to the lower affinity site revealed that only guanidinium or imidazoline derivatives compete with the specific binding of this radioligand. Our results suggest that [3H]cirazoline could be used as a novel ligand to label the non-adrenergic imidazoline-preferring sites.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0014-2999
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
24
|
|
pubmed:volume |
278
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
261-4
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:7589165-Animals,
pubmed-meshheading:7589165-Binding, Competitive,
pubmed-meshheading:7589165-Binding Sites,
pubmed-meshheading:7589165-Brain,
pubmed-meshheading:7589165-Guanidines,
pubmed-meshheading:7589165-Imidazoles,
pubmed-meshheading:7589165-Imidazoline Receptors,
pubmed-meshheading:7589165-Kidney,
pubmed-meshheading:7589165-Kinetics,
pubmed-meshheading:7589165-Male,
pubmed-meshheading:7589165-Membranes,
pubmed-meshheading:7589165-Phenethylamines,
pubmed-meshheading:7589165-Rats,
pubmed-meshheading:7589165-Rats, Sprague-Dawley,
pubmed-meshheading:7589165-Receptors, Drug,
pubmed-meshheading:7589165-Sensitivity and Specificity,
pubmed-meshheading:7589165-Tritium
|
|
pubmed:year |
1995
|
|
pubmed:articleTitle |
Binding of [3H]cirazoline to an imidazoline site in rat brain and kidney membranes.
|
|
pubmed:affiliation |
Synthelabo Research, Department of Preclinical Research, Bagneux, France.
|
|
pubmed:publicationType |
Journal Article
|
