Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1995-11-28
|
| pubmed:abstractText |
CD34, a stem cell marker, has been shown to be expressed on human CD3-CD4-CD8- (triple-negative; TN) thymocytes. Phenotypic and functional analyses suggest the following differentiation sequence: CD34+1-3-4-8(-)--> CD34+1+3-4 +/- 8(-)-->CD34-1+3-4+8(+/-)-->CD34-1++3-4+8+. In this report, we examined cytokine receptor gene expression on these subsets by reverse transcription-polymerase chain reaction analysis (RT-PCR). We were able to detect interleukin-7 receptor (IL-7R), c-kit and IL-2R gamma in all CD34+ thymocyte subsets, consistent with previous functional studies. We found IL-1R, granulocyte/macrophage colony-stimulating factor receptor-alpha and IL-4R transcripts in CD3- and CD34+ subsets. Secondly, we investigated T cell receptor (TCR)-delta and -beta gene rearrangement and transcription in CD34+ thymocytes. Our results show that a full-length TCR-delta transcript and the recombination activating genes RAG-1 and RAG-2 mRNA were already expressed in the CD34+1- subset. Mature V beta-containing TCR transcripts were also detected in the CD34+1+ subset, but not in the CD1- fraction. Furthermore, PCR analysis of D-J beta gene rearrangements showed that > or = 70% of CD34+1- cells are in a TCR beta germ-line configuration, although D-J beta recombination had already started in this population.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0014-2980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
25
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2471-8
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7589113-Antigens, CD34,
pubmed-meshheading:7589113-Base Sequence,
pubmed-meshheading:7589113-Cells, Cultured,
pubmed-meshheading:7589113-Child,
pubmed-meshheading:7589113-Child, Preschool,
pubmed-meshheading:7589113-DNA,
pubmed-meshheading:7589113-Gene Expression,
pubmed-meshheading:7589113-Humans,
pubmed-meshheading:7589113-Molecular Sequence Data,
pubmed-meshheading:7589113-Polymerase Chain Reaction,
pubmed-meshheading:7589113-Receptors, Antigen, T-Cell,
pubmed-meshheading:7589113-Receptors, Cytokine,
pubmed-meshheading:7589113-Thymus Gland
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
CD34-positive early human thymocytes: T cell receptor and cytokine receptor gene expression.
|
| pubmed:affiliation |
Laboratoire d'Immunologie Cellulaire et Tissulaire, CNRS URA 625, Hôpital de la Pitié-Salpétrière, Paris, France.
|
| pubmed:publicationType |
Journal Article
|