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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1995-12-14
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pubmed:abstractText |
The CYP17 gene contains in its promoter region at least two cis-acting elements (cAMP-responsive sequence 1 and 2, CRS1 and CRS2) that are necessary for adrenocorticotropin (ACTH) induced transcription. The CRS2 element contains a 6 bp repeat similar to binding sites for members of the nuclear hormone receptor superfamily of transcription factors. We present data that establish the repeated part of CRS2 (repCRS2) as a target of two nuclear orphan receptors; steroidogenic factor 1 (SF-1) and chicken ovalbumin upstream promoter transcription factor (COUP-TF). The repCRS2 element was found to form COUP-TF-related complexes with nuclear extracts from all cell lines tested, whereas SF-1-related complexes were only formed with extracts from steroidogenic Y1 cells. Transfection studies of steroidogenic cells demonstrated that SF-1 acts as an activator of repCRS2-dependent transcription of reporter genes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/COUP Transcription Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fushi Tarazu Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NR2F1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/NR5A1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nr2f1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glucocorticoid,
http://linkedlifedata.com/resource/pubmed/chemical/Steroidogenic Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/steroidogenic factor 1, mouse
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pubmed:status |
MEDLINE
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pubmed:issn |
0743-5800
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
509-16
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pubmed:dateRevised |
2009-8-12
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pubmed:meshHeading |
pubmed-meshheading:7588416-Animals,
pubmed-meshheading:7588416-Base Sequence,
pubmed-meshheading:7588416-COUP Transcription Factor I,
pubmed-meshheading:7588416-Cattle,
pubmed-meshheading:7588416-DNA-Binding Proteins,
pubmed-meshheading:7588416-Fushi Tarazu Transcription Factors,
pubmed-meshheading:7588416-Gene Expression Regulation,
pubmed-meshheading:7588416-Homeodomain Proteins,
pubmed-meshheading:7588416-Humans,
pubmed-meshheading:7588416-Mice,
pubmed-meshheading:7588416-Molecular Sequence Data,
pubmed-meshheading:7588416-Protein Binding,
pubmed-meshheading:7588416-Protein Biosynthesis,
pubmed-meshheading:7588416-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:7588416-Receptors, Glucocorticoid,
pubmed-meshheading:7588416-Steroidogenic Factor 1,
pubmed-meshheading:7588416-Transcription, Genetic,
pubmed-meshheading:7588416-Transcription Factors,
pubmed-meshheading:7588416-Tumor Cells, Cultured
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pubmed:articleTitle |
Transcriptional regulation of the bovine CYP17 gene: two nuclear orphan receptors determine activity of cAMP-responsive sequence 2.
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pubmed:affiliation |
Department of Medical Nutrition, Karolinska Institute, Huddinge University Hospital, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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