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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-12-21
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pubmed:abstractText |
Human pregnancy-associated plasma protein A (PAPP-A) inhibited significantly the proteolytic activity of bovine trypsin and human plasmin. Trypsin or plasmin treatment of PAPP-A resulted in the generation of a major 85 kDa component and the rapid cleavage of internal thiol esters. The results indicated that both of these serine proteinases bound in a 1:1 stoichiometry to PAPP-A. The PAPP-A-bound enzymes were found to be enzymatically active towards small synthetic substrates and inaccessible to inactivation by soybean trypsin inhibitor and alpha 1-proteinase inhibitor. The mechanism of proteinase inhibition was likely to be entrapment, as described for alpha 2-macroglobulin.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0009-8981
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
239
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
47-55
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7586586-Amino Acid Sequence,
pubmed-meshheading:7586586-Animals,
pubmed-meshheading:7586586-Cattle,
pubmed-meshheading:7586586-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7586586-Fibrinolysin,
pubmed-meshheading:7586586-Humans,
pubmed-meshheading:7586586-Molecular Sequence Data,
pubmed-meshheading:7586586-Plant Proteins,
pubmed-meshheading:7586586-Pregnancy-Associated Plasma Protein-A,
pubmed-meshheading:7586586-Protease Inhibitors,
pubmed-meshheading:7586586-Serine Proteinase Inhibitors,
pubmed-meshheading:7586586-Trypsin,
pubmed-meshheading:7586586-Trypsin Inhibitors,
pubmed-meshheading:7586586-alpha 1-Antitrypsin,
pubmed-meshheading:7586586-alpha-Amylases
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pubmed:year |
1995
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pubmed:articleTitle |
Interaction of human pregnancy-associated plasma protein-A with serine proteinases.
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pubmed:affiliation |
Central Research Laboratory, Postgraduate Physician Training Institute, Novokuznetsk, Russian Federation.
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pubmed:publicationType |
Journal Article
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