Linked Life Data

7585894

Source:http://linkedlifedata.com/resource/pubmed/id/7585894

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-12-13
pubmed:abstractText
Effects of external ATP application on the intracellular Ca2+ concentration ([Ca2+]i) of the epithelial lining cells of the cochlear lateral wall, the stria vascularis (SV), spiral prominence (SP), and external sulcus (ES) cells, were examined by the fluorescent Ca2+ indicator, Fura-2. ATP induced an increase in [Ca2+]i of these epithelial cells loaded with Fura-2 in a dose-dependent manner (1-100 microM). The strongest response was observed in SP and ES cells, whereas SV cells showed a weak response. The increase in [Ca2+]i was a biphasic response consisting of a rapid transient peak followed by a sustained phase. Removal of the external Ca2+ caused a slight transient increase in [Ca2+]i without a subsequent sustained phase. The Mn2(+)-quenching method revealed the Ca2+ entry across the plasma membrane immediately after the ATP application. The initial peak results from both the release of Ca2+ from intracellular stores and the Ca2+ influx from the extracellular space. The sustained phase is totally derived from the external Ca2+. The effective order of purinergic agonists was 2-methylthio ATP > or = ATP > 3'-O-(4-benzoyl)benzoyl ATP > alpha, beta-methylene ATP > or = ADP, but adenosine or UTP showed no response. The ATP-induced [Ca2+]i response was inhibited by reactive blue 2. The [Ca2+]i was partially dependent on the concentration of the fully ionized form, ATP-4. These findings indicate the presence of both P2y- and P2z-purinergic receptors in the non-sensory epithelial cells of the lateral wall.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0143-4160
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
89-99
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Calcium mobilization and entry induced by extracellular ATP in the non-sensory epithelial cell of the cochlear lateral wall.
pubmed:affiliation
Department of Otorhinolaryngology, Tohoku University School of Medicine, Sendai, Japan.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't