| pubmed-article:7584079 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7584079 | lifeskim:mentions | umls-concept:C0005955 | lld:lifeskim |
| pubmed-article:7584079 | lifeskim:mentions | umls-concept:C0162371 | lld:lifeskim |
| pubmed-article:7584079 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:7584079 | lifeskim:mentions | umls-concept:C0017768 | lld:lifeskim |
| pubmed-article:7584079 | lifeskim:mentions | umls-concept:C1332710 | lld:lifeskim |
| pubmed-article:7584079 | lifeskim:mentions | umls-concept:C1160185 | lld:lifeskim |
| pubmed-article:7584079 | lifeskim:mentions | umls-concept:C0206243 | lld:lifeskim |
| pubmed-article:7584079 | lifeskim:mentions | umls-concept:C1514926 | lld:lifeskim |
| pubmed-article:7584079 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:7584079 | pubmed:dateCreated | 1995-12-27 | lld:pubmed |
| pubmed-article:7584079 | pubmed:abstractText | One promising strategy for gene therapy of Gaucher disease involves ex vivo retroviral transduction of autologous hematopoietic stem cells. Studies in small animals have demonstrated that this approach provides a life-long supply of the glucocerebrosidase (GC) enzyme. Human application has developed to the stage of a clinical trial. In this study, we describe development of a high titer amphotropic producer line for the vector, MFG-GC, and explore transduction of CD34+ cells from various human sources. Higher than three times the normal levels of glucocerebrosidase activity in non-transduced cells were achieved following transduction of CD34+ cells obtained from bone marrow or cord blood from normal donors. The improvement in enzyme activity in Gaucher marrow was about 40-fold above deficient levels. We examined the timing and stepwise effect of multiple rounds of infection and evaluated post-infection expansion of cells in two different cytokine mixtures. Transduction efficiency was determined using immunocytochemistry and Southern blot hybridization. | lld:pubmed |
| pubmed-article:7584079 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7584079 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7584079 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7584079 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7584079 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7584079 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7584079 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7584079 | pubmed:month | May | lld:pubmed |
| pubmed-article:7584079 | pubmed:issn | 0969-7128 | lld:pubmed |
| pubmed-article:7584079 | pubmed:author | pubmed-author:FexJJ | lld:pubmed |
| pubmed-article:7584079 | pubmed:author | pubmed-author:OhashiTT | lld:pubmed |
| pubmed-article:7584079 | pubmed:author | pubmed-author:BoggsS SSS | lld:pubmed |
| pubmed-article:7584079 | pubmed:author | pubmed-author:BarrangerJ... | lld:pubmed |
| pubmed-article:7584079 | pubmed:author | pubmed-author:OOIS KSK | lld:pubmed |
| pubmed-article:7584079 | pubmed:author | pubmed-author:BallE DED | lld:pubmed |
| pubmed-article:7584079 | pubmed:author | pubmed-author:RobbinsP DPD | lld:pubmed |
| pubmed-article:7584079 | pubmed:author | pubmed-author:BahnsonA BAB | lld:pubmed |
| pubmed-article:7584079 | pubmed:author | pubmed-author:NimgaonkarMM | lld:pubmed |
| pubmed-article:7584079 | pubmed:author | pubmed-author:DuniganJJ | lld:pubmed |
| pubmed-article:7584079 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7584079 | pubmed:volume | 1 | lld:pubmed |
| pubmed-article:7584079 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7584079 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7584079 | pubmed:pagination | 176-84 | lld:pubmed |
| pubmed-article:7584079 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7584079 | pubmed:meshHeading | pubmed-meshheading:7584079-... | lld:pubmed |
| pubmed-article:7584079 | pubmed:meshHeading | pubmed-meshheading:7584079-... | lld:pubmed |
| pubmed-article:7584079 | pubmed:meshHeading | pubmed-meshheading:7584079-... | lld:pubmed |
| pubmed-article:7584079 | pubmed:meshHeading | pubmed-meshheading:7584079-... | lld:pubmed |
| pubmed-article:7584079 | pubmed:meshHeading | pubmed-meshheading:7584079-... | lld:pubmed |
| pubmed-article:7584079 | pubmed:meshHeading | pubmed-meshheading:7584079-... | lld:pubmed |
| pubmed-article:7584079 | pubmed:meshHeading | pubmed-meshheading:7584079-... | lld:pubmed |
| pubmed-article:7584079 | pubmed:meshHeading | pubmed-meshheading:7584079-... | lld:pubmed |
| pubmed-article:7584079 | pubmed:meshHeading | pubmed-meshheading:7584079-... | lld:pubmed |
| pubmed-article:7584079 | pubmed:meshHeading | pubmed-meshheading:7584079-... | lld:pubmed |
| pubmed-article:7584079 | pubmed:meshHeading | pubmed-meshheading:7584079-... | lld:pubmed |
| pubmed-article:7584079 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7584079 | pubmed:articleTitle | Transduction of CD34+ enriched cord blood and Gaucher bone marrow cells by a retroviral vector carrying the glucocerebrosidase gene. | lld:pubmed |
| pubmed-article:7584079 | pubmed:affiliation | Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, USA. | lld:pubmed |
| pubmed-article:7584079 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7584079 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:7584079 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7584079 | lld:pubmed |
