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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
1995-12-14
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pubmed:abstractText |
The effects of Ca2+ /phospholipid dependent (PKC) phosphorylation on the current amplitudes of alpha 1 and alpha 2 glycine receptors expressed in Xenopus oocytes were examined by whole cell voltage clamp recording. In studies using phorbol esters, PKC phosphorylation has been shown to reduce glycine-induced currents. Endogenous PKC activation by pretreatment with serum, however, enhanced the currents to around 140% in both alpha 1 and alpha 2 glycine receptors. This effect was completely blocked by a specific PKC inhibitor, GF109203X. Instead, treatment with a potent PKC activator, 12-O-tetradecanoylphorbol-13-acetate (TPA) revealed a decrease in glycine-gated channel currents. Thus, the present results demonstrate that glycine receptor phosphorylation mediated by endogenous pathway of PKC activation potentiates glycine-induced currents and phorbol esters may have a direct action on glycine receptor channels independent of PKC activation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glycine,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glycine,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-8993
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
687
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
214-6
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:7583309-Animals,
pubmed-meshheading:7583309-Enzyme Activation,
pubmed-meshheading:7583309-Glycine,
pubmed-meshheading:7583309-Ion Channels,
pubmed-meshheading:7583309-Oocytes,
pubmed-meshheading:7583309-Patch-Clamp Techniques,
pubmed-meshheading:7583309-Protein Kinase C,
pubmed-meshheading:7583309-RNA, Messenger,
pubmed-meshheading:7583309-Receptors, Glycine,
pubmed-meshheading:7583309-Tetradecanoylphorbol Acetate,
pubmed-meshheading:7583309-Xenopus
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pubmed:year |
1995
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pubmed:articleTitle |
Activation of endogenous protein kinase C enhances currents through alpha 1 and alpha 2 glycine receptor channels.
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pubmed:affiliation |
Department of Physiology, Kobe university School of Medicine, Japan.
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pubmed:publicationType |
Journal Article
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