Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1995-12-8
|
| pubmed:abstractText |
Polyacetylenic alcohols such as panaxytriol, panaxynol and panaxydol isolated from the roots of Panax ginseng C. A. MEYER have antiproliferative activity against various cultured tumor cells. Anti-panaxytriol antibody was obtained by immunizing rabbits with panaxytriol-bovine serum albumin conjugates. Although the antibody reactivity was directed mainly toward panaxytriol, there was a slight cross-reactivity with other polyacetylenic compounds. The antibody was, therefore, used for screening a large number of crude drugs for polyacetylenic compounds such as panaxytriol. Methanol-extracts from 31 crude drugs were examined. Significant reactivity was observed in 15 methanol-extracts from Aralieaceae, Compositae and Umbelliferae as reported by other investigators. Three out of the 15 crude drugs were selected for determination of the potent cross-reactive compounds. Four kinds of cross-reactive compounds were isolated by silica gel column chromatography, monitoring each fraction using the enzyme-linked immunosorbent assay (ELISA). Among them, panaxynol and heptadeca-1,8-diene-4,6-diyne-3,10-diol were identified from Saposhnikoviae Radix. Falcarindiol was newly identified from Peucedani Radix. A new polyacetylenic alcohol, 9,10-epoxy-16-hydroxy-octadeca-17-ene-12,14-diyne-1-al, was also isolated from Foeniculi Fructus. All these polyacetylenic alcohols inhibited the growth of a human gastric adenocarcinoma cell line, MK-1 cells, in a dose-dependent manner. These results indicate that the antibody against panaxytriol is an effective tool for "screening" antiproliferative polyacetylenic compounds.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alkynes,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents, Phytogenic,
http://linkedlifedata.com/resource/pubmed/chemical/Drugs, Chinese Herbal,
http://linkedlifedata.com/resource/pubmed/chemical/Enediynes,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Alcohols,
http://linkedlifedata.com/resource/pubmed/chemical/panaxytriol
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0918-6158
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
18
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
933-7
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7581246-Adenocarcinoma,
pubmed-meshheading:7581246-Alkynes,
pubmed-meshheading:7581246-Animals,
pubmed-meshheading:7581246-Antibody Specificity,
pubmed-meshheading:7581246-Antineoplastic Agents, Phytogenic,
pubmed-meshheading:7581246-Cross Reactions,
pubmed-meshheading:7581246-Drug Screening Assays, Antitumor,
pubmed-meshheading:7581246-Drugs, Chinese Herbal,
pubmed-meshheading:7581246-Enediynes,
pubmed-meshheading:7581246-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:7581246-Fatty Alcohols,
pubmed-meshheading:7581246-Humans,
pubmed-meshheading:7581246-Mice,
pubmed-meshheading:7581246-Mice, Nude,
pubmed-meshheading:7581246-Neoplasm Transplantation,
pubmed-meshheading:7581246-Spectrophotometry, Infrared,
pubmed-meshheading:7581246-Stomach Neoplasms,
pubmed-meshheading:7581246-Tumor Cells, Cultured,
pubmed-meshheading:7581246-Tumor Stem Cell Assay
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Screening of polyacetylenic alcohols in crude drugs using the ELISA for panaxytriol.
|
| pubmed:affiliation |
Faculty of Hospital Pharmacy, Saga Medical School, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|