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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1995-11-29
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pubmed:databankReference |
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pubmed:abstractText |
A polymerase chain reaction assay (Lust J.A. et al. (1992). Cytokine 4:96-100) was used to detect a mRNA coding for a soluble IL-6 receptor in human hepatoma cells. In addition to the expected amplification product, we found a sequence (SR4) which could be aligned to it with 78% identity. After cloning and sequencing a genomic 2.5-2.7 kB EcoRI fragment containing SR4, this sequence turned out to be part of a pseudogene corresponding to the transmembrane domain deleted soluble IL-6 receptor. Screening of a panel of interspecies hybrids revealed that it maps to chromosome 9.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1042-5179
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:geneSymbol |
IL-6R,
SR4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
311-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7579586-Antigens, CD,
pubmed-meshheading:7579586-Base Sequence,
pubmed-meshheading:7579586-Blotting, Southern,
pubmed-meshheading:7579586-Carcinoma, Hepatocellular,
pubmed-meshheading:7579586-Chromosome Mapping,
pubmed-meshheading:7579586-Chromosomes, Human, Pair 9,
pubmed-meshheading:7579586-Cloning, Molecular,
pubmed-meshheading:7579586-DNA Primers,
pubmed-meshheading:7579586-Genomic Library,
pubmed-meshheading:7579586-Humans,
pubmed-meshheading:7579586-Molecular Sequence Data,
pubmed-meshheading:7579586-Polymerase Chain Reaction,
pubmed-meshheading:7579586-Pseudogenes,
pubmed-meshheading:7579586-RNA, Messenger,
pubmed-meshheading:7579586-Receptors, Interleukin,
pubmed-meshheading:7579586-Receptors, Interleukin-6,
pubmed-meshheading:7579586-Sequence Alignment,
pubmed-meshheading:7579586-Tumor Cells, Cultured
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pubmed:year |
1995
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pubmed:articleTitle |
Cloning and chromosomal localization of a pseudogene corresponding to a mRNA for a soluble IL-6 receptor.
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pubmed:affiliation |
Institut Pasteur du Brabant, Bruxelles, Belgium.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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