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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0024264,
umls-concept:C0086418,
umls-concept:C0205307,
umls-concept:C0521447,
umls-concept:C0812382,
umls-concept:C1171362,
umls-concept:C1420642,
umls-concept:C1515670,
umls-concept:C1709160,
umls-concept:C2700640
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pubmed:issue |
8
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pubmed:dateCreated |
1995-11-29
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pubmed:abstractText |
The TCF-1 gene encodes a putative transcription factor with affinity for a sequence motif occurring in a number of T-cell enhancers. TCF-1 mRNA was originally found to be expressed in a T cell-specific fashion within a set of human and mouse cell lines. In contrast, expression reportedly occurs in multiple nonlymphoid tissues during murine embryogenesis. We have now raised a monoclonal antibody to document expression and biochemistry of the human TCF-1 protein. As expected, the TCF-1 protein was detectable only in cell lines of T lineage. Its expression was always restricted to the nucleus. Immunohistochemistry on a panel of human tissues revealed that the TCF-1 protein was found exclusively in thymocytes and in CD3+ T cells in peripheral lymphoid tissues. Western blotting yielded a set of bands ranging from 25 kD to 55 kD, resulting from extensive alternative splicing. The TCF-1 protein was detectable in all samples of a set of 22 T-cell malignancies of various stages of maturation, but was absent from a large number of other hematologic neoplasms. These observations imply a T cell-specific function for TCF-1, a notion corroborated by recent observations on Tcf-1 knock-out mice. In addition, these results indicate that nuclear TCF-1 expression can serve as a pan-T-lineage marker in the diagnosis of lymphoid malignancies.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphoid Enhancer-Binding Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/T Cell Transcription Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/TCF7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tcf7 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3050-9
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:7579399-Amino Acid Sequence,
pubmed-meshheading:7579399-Animals,
pubmed-meshheading:7579399-Base Sequence,
pubmed-meshheading:7579399-Biological Markers,
pubmed-meshheading:7579399-DNA-Binding Proteins,
pubmed-meshheading:7579399-Genes,
pubmed-meshheading:7579399-Humans,
pubmed-meshheading:7579399-Injections, Subcutaneous,
pubmed-meshheading:7579399-Leukemia, T-Cell,
pubmed-meshheading:7579399-Lymphoid Enhancer-Binding Factor 1,
pubmed-meshheading:7579399-Lymphoma, T-Cell,
pubmed-meshheading:7579399-Mice,
pubmed-meshheading:7579399-Mice, Inbred BALB C,
pubmed-meshheading:7579399-Molecular Sequence Data,
pubmed-meshheading:7579399-Neoplasm Proteins,
pubmed-meshheading:7579399-RNA Splicing,
pubmed-meshheading:7579399-Recombinant Fusion Proteins,
pubmed-meshheading:7579399-T Cell Transcription Factor 1,
pubmed-meshheading:7579399-T-Lymphocytes,
pubmed-meshheading:7579399-Transcription Factors,
pubmed-meshheading:7579399-Tumor Markers, Biological
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pubmed:year |
1995
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pubmed:articleTitle |
The human TCF-1 gene encodes a nuclear DNA-binding protein uniquely expressed in normal and neoplastic T-lineage lymphocytes.
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pubmed:affiliation |
Department of Immunology, University Hospital Utrecht, The Netherlands.
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pubmed:publicationType |
Journal Article
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