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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1995-11-2
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pubmed:abstractText |
The hemagglutinin (HA) glycoprotein encoded by measles virus (MV) is a type II integral membrane protein that is expressed at the infected cell surface. Genes encoding wild-type MV HA as well as two mutant HA proteins shortened at their carboxy-termini by either 18 (HA delta 18O) or 223 (HA delta 223) amino acids were constructed and studied in a transient expression system in COS cells. Under nonreducing conditions, assembly of HA delta 18 into homodimers was diminished while HA delta 223 remained in a monomeric form. Hemadsorption assays revealed that neither mutant was functional at the cell surface. These studies show that the carboxy-terminal ectodomain of the HA protein is essential to its proper folding and assembly into homodimers while its carboxy-terminal 18 amino acids are essential for the hemadsorption (receptor-binding) function of the protein.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
214
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1232-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7575535-Animals,
pubmed-meshheading:7575535-Base Sequence,
pubmed-meshheading:7575535-Cell Line,
pubmed-meshheading:7575535-Cercopithecus aethiops,
pubmed-meshheading:7575535-Chymotrypsin,
pubmed-meshheading:7575535-Erythrocytes,
pubmed-meshheading:7575535-Hemagglutination,
pubmed-meshheading:7575535-Hemagglutinins, Viral,
pubmed-meshheading:7575535-Kidney,
pubmed-meshheading:7575535-Kinetics,
pubmed-meshheading:7575535-Macromolecular Substances,
pubmed-meshheading:7575535-Measles virus,
pubmed-meshheading:7575535-Molecular Sequence Data,
pubmed-meshheading:7575535-Mutagenesis, Site-Directed,
pubmed-meshheading:7575535-Peptide Mapping,
pubmed-meshheading:7575535-Protein Folding,
pubmed-meshheading:7575535-Recombinant Proteins,
pubmed-meshheading:7575535-Sequence Deletion,
pubmed-meshheading:7575535-Transfection,
pubmed-meshheading:7575535-Vero Cells
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pubmed:year |
1995
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pubmed:articleTitle |
The carboxy-terminal 18 amino acids of the measles virus hemagglutinin are essential for its biological function.
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pubmed:affiliation |
Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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