Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-11-2
pubmed:abstractText
Histamine H2 receptors were tagged at the N-terminus with the eight amino acid Flag epitope to allow the immunological identification of the receptor peptide with the monoclonal anti-Flag M2 antibody. The introduction of the epitope did not modify the binding of several H2 ligands to the H2 receptor, nor the ability of histamine to stimulate the H2 receptor mediated cAMP production in HEK-293 cells. Western blots revealed a major protein band of 57 +/- 1 kDa, whereas a second band of 31 +/- 1 kDa was probably the result of a proteolytic breakdown of the 57 kDa band. Immunofluorescence measurements of stably transfected HEK-293 cells revealed the presence of anti-Flag-immunoreactivity in the plasma membrane. This immunoreactivity completely disappeared after a one hour treatment with histamine. The receptor internalization was reversible and blocked by the endocytosis inhibitor phenylarsine oxide. Forskolin did not induce H2 receptor internalization, indicating that histamine causes H2 receptor internalization via a cAMP-independent pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
214
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1138-45
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7575521-Amino Acid Sequence, pubmed-meshheading:7575521-Animals, pubmed-meshheading:7575521-Base Sequence, pubmed-meshheading:7575521-Blotting, Western, pubmed-meshheading:7575521-Cell Line, pubmed-meshheading:7575521-DNA Primers, pubmed-meshheading:7575521-Epitopes, pubmed-meshheading:7575521-Fluorescent Antibody Technique, pubmed-meshheading:7575521-Histamine, pubmed-meshheading:7575521-Histamine Agonists, pubmed-meshheading:7575521-Humans, pubmed-meshheading:7575521-Kidney, pubmed-meshheading:7575521-Kinetics, pubmed-meshheading:7575521-Molecular Sequence Data, pubmed-meshheading:7575521-Polymerase Chain Reaction, pubmed-meshheading:7575521-Radioligand Assay, pubmed-meshheading:7575521-Rats, pubmed-meshheading:7575521-Receptors, Histamine H2, pubmed-meshheading:7575521-Recombinant Proteins, pubmed-meshheading:7575521-Transfection
pubmed:year
1995
pubmed:articleTitle
Visualization of agonist-induced internalization of histamine H2 receptors.
pubmed:affiliation
Leiden/Amsterdam Center for Drug Research, Department of Pharmacochemistry, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't