Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1995-11-14
pubmed:abstractText
DNA from 32 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria from diverse locations was probed with the first three genes of the well-known 2,4-D degradation pathway found in Alcaligenes eutrophus JMP134(pJP4). The majority of strains did not show high levels of homology to the first three genes of the 2,4-D degradation pathway, tfdA, -B, and -C. Most strains showed combinations of tfdA-, B-, and C-like elements that exhibited various degrees of homology to the gene probes. Strains having the same genomic fingerprints (as determined by repetitive extragenic palindromic PCR) exhibited the same hybridization pattern regardless of the geographic origin of the strain, with the exception of a strain isolated from Puerto Rico. This strain had the same genomic fingerprint as that of numerous other strains in the collection but differed in its hybridization against the tfdA gene probe. Members of the beta subdivision of the Proteobacteria class, specifically Alcaligenes, Burkholderia, and Rhodoferax species, carried DNA fragments with 60% or more sequence similarity to tfdA of pJP4, and most carried fragments showing at least 60% homology to tfdB. However, many strains did not hybridize with tfdC, although they exhibited chlorocatechol dioxygenase activity. Members of the alpha subdivision of the Proteobacteria class, mostly of the genus Sphingomonas, did not hybridize to either tfdA or tfdC, but some hybridized at low stringency to tfdB. The data suggest that extensive interspecies transfer of a variety of homologous degradative genes has been involved in the evolution of 2,4-D-degrading bacteria.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-1311543, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-1325141, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-1389314, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-1429434, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-1476437, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-1480115, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-1650730, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-1656436, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-1732207, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-1762913, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-1987160, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-1993181, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-2013566, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-2115872, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-2185214, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-2231712, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-2244784, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-2403263, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-2413450, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-2604403, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-2830457, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-2830460, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-2842290, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-2914848, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-2981813, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-3036764, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-3203254, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-3707123, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-3923930, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-6223610, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-6257648, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-697765, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-697766, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-7509586, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-7646006, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-8017907, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-8226980, http://linkedlifedata.com/resource/pubmed/commentcorrection/7574638-8332524
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3274-81
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
2,4-Dichlorophenoxyacetic acid-degrading bacteria contain mosaics of catabolic genes.
pubmed:affiliation
Center for Microbial Ecology, Michigan State University, East Lansing 48824-1101, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.