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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1995-11-20
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pubmed:abstractText |
The quantitation of B lymphocytes in paraffin-decalcified bone marrow sections is often used as supportive evidence of primary or residual B-cell lymphoproliferative disorders; however, well-defined normal ranges for B lymphocytes are not available. Thirty-four B5-fixed decalcified bone marrow sections were analyzed from a group of patients having an essentially normal bone marrow morphologic examination and lacking any evidence immunologic or hematopoietic disease. All samples were stained with CD20, CD45RA (4KB5), and DBA.44 for B cells, and CD45RO (UCHL-1) for T cells. Image analysis was performed on all cases. CD20-positive cells ranged from 0% to 5.97%. CD45RA positivity ranged from 0% to 5.48%. DBA.44 was positive in 0% to 2.07% of the cells. CD45RO positivity varied from 0% to 6.7%. These results provide useful immunohistochemical B-lymphocyte reference ranges in B5-fixed paraffin bone marrow sections.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0002-9173
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
104
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
517-23
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:7572811-Adult,
pubmed-meshheading:7572811-Aged,
pubmed-meshheading:7572811-B-Lymphocytes,
pubmed-meshheading:7572811-Biopsy, Needle,
pubmed-meshheading:7572811-Bone Marrow Cells,
pubmed-meshheading:7572811-Female,
pubmed-meshheading:7572811-Humans,
pubmed-meshheading:7572811-Immunohistochemistry,
pubmed-meshheading:7572811-Male,
pubmed-meshheading:7572811-Middle Aged,
pubmed-meshheading:7572811-Paraffin,
pubmed-meshheading:7572811-Reference Values
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pubmed:year |
1995
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pubmed:articleTitle |
Immunohistochemical reference ranges for B lymphocytes in bone marrow biopsy paraffin sections.
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pubmed:affiliation |
Saint Mary's Medical Center, Saginaw, Michigan, USA.
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pubmed:publicationType |
Journal Article
|