Linked Life Data

7572398

Source:http://linkedlifedata.com/resource/pubmed/id/7572398

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1995-11-14
pubmed:abstractText
Infection of quiescent lymphocytes with human immunodeficiency virus type 1 (HIV-1) does not result in production of progeny virus. We have previously reported that although HIV-1 can enter quiescent lymphocytes with high efficiency, the reverse transcription process does not go to completion. This results in a viral genome which is composed partly of viral RNA and partly of viral DNA. If a mitogenic signal is applied shortly after infection to a cell harboring such a structure, reverse transcription can go to completion and progeny virus will be produced. However, this partially reverse transcribed structure is extremely labile, and the efficiency of virus rescue decreases rapidly, with increasing times between infection and activation. Our laboratory is using inhibitors of cell activation to identify at which stage of the cell cycle this block to reverse transcription occurs. We have found that agents that arrest the cell in the late G1 phase of the cell cycle do not alter the ability of the virus to complete reverse transcription. However, agents that inhibit activation of the cell by blocking transition through G1 prevent completion of reverse transcription. It thus appears that immunosuppression of the target cell may be a means of preventing productive infection of the cell. We have also been using the severe combined immunodeficient mouse implanted with human tissue (SCID-hu) as an in vivo model to study HIV-1 pathogenic properties. When human fetal thymic implants in these animals are infected by HIV-1, profound depletion of CD4-bearing human thymocytes is seen.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0065-2598
pubmed:author
pubmed:issnType
Print
pubmed:volume
374
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
27-31
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
The role of the cell cycle in HIV-1 infection.
pubmed:affiliation
Department of Medicine, UCLA School of Medicine, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Review