Linked Life Data

757098

Source:http://linkedlifedata.com/resource/pubmed/id/757098

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1980-2-15
pubmed:abstractText
Rat hypophyseal intermediate lobe cells, partially dispersed by trypsin, were cultivated on an inert surface. The attaching capacity, the growth of the cultures and the fine structure of the epithelial cells developed during cultivation were studied. The majority of the cell groups attached to the inert surface within 72 h after explantation. Epithelial islands developed, with a layer consisting of fibroblast-like cells beginning to form at their periphery. By the end of the second week of cultivation the epithelial islets and fibroblast-like cells formed a monolayer. The epithelial cells survived for five weeks. The granular cytoplasm of the epithelial island cells could be well identified on the phase-contrast microcinematographic frames of the young cultures; both epithelial and fibroblast-like cells divided. The mitotic ability of both cell types could be confirmed by their tritiated-thymidine incorporation, too. In young cultures, the fine-structural features of epithelial cells were practically identical with those of the glandular cells in intact glands. In older cultures, the secretory granules in the epithelial cells were lower in number; the localization of the granules at the periphery was predominating.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0001-5288
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
273-83
pubmed:dateRevised
2005-7-1
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Monolayer cultures of rat pituitary intermediate lobe: growth and fine structure of epithelial cells.
pubmed:publicationType
Journal Article