Linked Life Data

7568495

Source:http://linkedlifedata.com/resource/pubmed/id/7568495

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-10-30
pubmed:abstractText
Syndactyly is a common congenital hand anomaly that may occur after exposure to teratogens. We have developed an in vitro model of syndactyly to investigate the molecular mechanisms underlying this malformation of digit development. Retinoic acid, which regulates pattern formation in vertebrate limb development and is associated with teratogenic malformations, was used in the development of this syndactyly model system. Pregnant Swiss-Webster mice were given retinoic acid by oral gavage on days 10 and 11 of embryonic development (E10 and E11, respectively). The mice were sacrificed on gestational days 13 and 17 (E13, E17) and immediately postnatally (PN). The fetuses were removed and the forelimbs dissected under the operating microscope. The E13 limbs were cultured for 4 days (E13+4) in an organ culture system using a serumless, chemically defined medium. The E17, PN, and E13+4 forelimbs were critically examined for malformations of digit separation and digit development. Retinoic acid-induced fetal mouse forelimb syndactyly was observed in all the groups; 81 percent of E17 limbs, 75 percent of PN limbs, and 77 percent of E13+4 limbs had syndactyly. The morphology of the digital malformations was similar in the E17, PN, and E13+4 limbs. This in vitro model permits further studies to characterize the molecular changes that occur during the development of a congenital hand anomaly.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0032-1052
pubmed:author
pubmed:issnType
Print
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1169-76
pubmed:dateRevised
2011-2-16
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
In vitro model of syndactyly replicates the morphologic features observed in vivo.
pubmed:affiliation
Department of Surgery, King-Drew Medical Center, Baylor College of Medicine, Houston, Texas, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't