| pubmed-article:7568021 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7568021 | lifeskim:mentions | umls-concept:C1643204 | lld:lifeskim |
| pubmed-article:7568021 | lifeskim:mentions | umls-concept:C1708096 | lld:lifeskim |
| pubmed-article:7568021 | lifeskim:mentions | umls-concept:C1444754 | lld:lifeskim |
| pubmed-article:7568021 | lifeskim:mentions | umls-concept:C0002085 | lld:lifeskim |
| pubmed-article:7568021 | lifeskim:mentions | umls-concept:C1524062 | lld:lifeskim |
| pubmed-article:7568021 | lifeskim:mentions | umls-concept:C1882417 | lld:lifeskim |
| pubmed-article:7568021 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:7568021 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:7568021 | lifeskim:mentions | umls-concept:C0205309 | lld:lifeskim |
| pubmed-article:7568021 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:7568021 | pubmed:dateCreated | 1995-10-23 | lld:pubmed |
| pubmed-article:7568021 | pubmed:abstractText | We describe a technique for HLA-Cw genotyping by digestion of PCR-amplified genes with restriction endonucleases. Locus-specific primers selectively amplified HLA-Cw sequences from exon 2 in a single PCR that avoided coamplification of other classical and nonclassical class I genes. Amplified DNAs were digested with selected enzymes. Sixty-three homozygous cell lines from International Histocompatibility Workshop X and 113 unrelated individual cells were genotypes for HLA-Cw and compared with serology. The present protocol can distinguish 23 alleles corresponding to the known HLA-Cw sequences. Genotyping of serologically undetectable alleles (HLA-Cw Blank) and of heterozygous cells was made possible by using this method. Six additional HLA-Cw alleles were identified by unusual restriction patterns and confirmed by sequencing; this observation suggests the presence of another family of allele-sharing clusters in the HLA-B locus. This PCR-restriction endonuclease method provides a simple and convenient approach for HLA-Cw DNA typing, allowing the definition of serologically undetectable alleles, and will contribute to the evaluation of the biological role of the HLA-C locus. | lld:pubmed |
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| pubmed-article:7568021 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7568021 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7568021 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7568021 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7568021 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:7568021 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:7568021 | pubmed:author | pubmed-author:LoiseauPP | lld:pubmed |
| pubmed-article:7568021 | pubmed:author | pubmed-author:CharronDD | lld:pubmed |
| pubmed-article:7568021 | pubmed:author | pubmed-author:RaffouxCC | lld:pubmed |
| pubmed-article:7568021 | pubmed:author | pubmed-author:FortierCC | lld:pubmed |
| pubmed-article:7568021 | pubmed:author | pubmed-author:TataruVV | lld:pubmed |
| pubmed-article:7568021 | pubmed:author | pubmed-author:BobryninaVV | lld:pubmed |
| pubmed-article:7568021 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7568021 | pubmed:day | 12 | lld:pubmed |
| pubmed-article:7568021 | pubmed:volume | 92 | lld:pubmed |
| pubmed-article:7568021 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7568021 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7568021 | pubmed:pagination | 8803-7 | lld:pubmed |
| pubmed-article:7568021 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:7568021 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7568021 | pubmed:articleTitle | HLA-Cw allele analysis by PCR-restriction fragment length polymorphism: study of known and additional alleles. | lld:pubmed |
| pubmed-article:7568021 | pubmed:affiliation | Laboratoire d'Immunologie et d'Histocompatibilité, Hôpital Saint Louis, Paris, France. | lld:pubmed |
| pubmed-article:7568021 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7568021 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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