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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1995-10-23
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pubmed:abstractText |
We describe a technique for HLA-Cw genotyping by digestion of PCR-amplified genes with restriction endonucleases. Locus-specific primers selectively amplified HLA-Cw sequences from exon 2 in a single PCR that avoided coamplification of other classical and nonclassical class I genes. Amplified DNAs were digested with selected enzymes. Sixty-three homozygous cell lines from International Histocompatibility Workshop X and 113 unrelated individual cells were genotypes for HLA-Cw and compared with serology. The present protocol can distinguish 23 alleles corresponding to the known HLA-Cw sequences. Genotyping of serologically undetectable alleles (HLA-Cw Blank) and of heterozygous cells was made possible by using this method. Six additional HLA-Cw alleles were identified by unusual restriction patterns and confirmed by sequencing; this observation suggests the presence of another family of allele-sharing clusters in the HLA-B locus. This PCR-restriction endonuclease method provides a simple and convenient approach for HLA-Cw DNA typing, allowing the definition of serologically undetectable alleles, and will contribute to the evaluation of the biological role of the HLA-C locus.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-1317015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-1383381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-1384166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-1541831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-1548148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-1589035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-1680839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-1683028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-1694227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-1968291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-1979875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-2115762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-2247120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-2315309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-2341305,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-2443855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-2576477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-2592018,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-2732471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-2787363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-2914713,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7568021-8493555
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8803-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7568021-Alleles,
pubmed-meshheading:7568021-B-Lymphocytes,
pubmed-meshheading:7568021-Base Sequence,
pubmed-meshheading:7568021-Cell Line,
pubmed-meshheading:7568021-European Continental Ancestry Group,
pubmed-meshheading:7568021-Exons,
pubmed-meshheading:7568021-Gene Frequency,
pubmed-meshheading:7568021-Genotype,
pubmed-meshheading:7568021-HLA-C Antigens,
pubmed-meshheading:7568021-Heterozygote,
pubmed-meshheading:7568021-Histocompatibility Testing,
pubmed-meshheading:7568021-Homozygote,
pubmed-meshheading:7568021-Humans,
pubmed-meshheading:7568021-Molecular Sequence Data,
pubmed-meshheading:7568021-Polymerase Chain Reaction,
pubmed-meshheading:7568021-Polymorphism, Restriction Fragment Length
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pubmed:year |
1995
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pubmed:articleTitle |
HLA-Cw allele analysis by PCR-restriction fragment length polymorphism: study of known and additional alleles.
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pubmed:affiliation |
Laboratoire d'Immunologie et d'Histocompatibilité, Hôpital Saint Louis, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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