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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
1995-11-6
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pubmed:abstractText |
The sequence-specific formation of R-loops can be assayed using RNAs which overlap a HindIII cleavage site in a 3.5 kb plasmid. Chemical modification of the displaced DNA strand has permitted stabilization of these R-loops and allowed a systematic investigation of the dependence of these triple-stranded structures on the chain length and structure of the input RNA. RNAs as short as 50 nt form stable R-loops if 5-allylamine uridines (Uaa-RNA) are used in place of normal uridines; normal RNAs must be 100 nt long to form R-loops quantitatively. Since acetic anhydride decreases the hybridization efficiency of Uaa-RNAs, the positive charge of the RNAs must diminish the electrostatic repulsion of the three negatively charged phosphodiester backbones. The dependence of R-loop stability on the length of RNA can be stimulated with a random walk model, which also applies to strand migration within Holiday junctions. R-loop hybridization provides a versatile method to generate single-stranded DNA in a sequence-selective manner.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-269380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-379820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-379821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-3917855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-396442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-5137601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-7506932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-7683427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-7684825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-7684856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-781674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-8021241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-8047884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-8134343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-8465200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7567464-902310
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3516-23
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7567464-DNA,
pubmed-meshheading:7567464-DNA, Single-Stranded,
pubmed-meshheading:7567464-Glyoxal,
pubmed-meshheading:7567464-Nucleic Acid Denaturation,
pubmed-meshheading:7567464-Nucleic Acid Hybridization,
pubmed-meshheading:7567464-Oligodeoxyribonucleotides,
pubmed-meshheading:7567464-Plasmids,
pubmed-meshheading:7567464-RNA,
pubmed-meshheading:7567464-Restriction Mapping,
pubmed-meshheading:7567464-Structure-Activity Relationship
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pubmed:year |
1995
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pubmed:articleTitle |
R-loop stability as a function of RNA structure and size.
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pubmed:affiliation |
Department of Biological Chemistry, School of Medicine, University of California-Los Angeles 90024-1570, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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