|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1995-10-26
|
|
pubmed:abstractText |
Positron-emitting radionuclides have short half-lives and high radiation energies compared with radioisotopes generally used in biomedical research. We examined the possibility of applying positron emitter-labeled compounds to functional imaging in brain slices kept viable in an oxygenated buffer solution. Brain slices (300 microns thick) containing the striatum were incubated with positron emitter-labeled tracers for 30-45 min. The slices were then rinsed and placed on the bottom of a Plexiglas chamber filled with oxygenated Krebs-Ringer solution. The bottom of the chamber consisted of a thin polypropylene film to allow good penetration of beta+ particles from the brain slices. The chamber was placed on a storage phosphor screen, which has a higher sensitivity and a wider dynamic range than X-ray films. After an exposure period of 15-60 min, the screen was scanned by the analyzer and radioactivity images of brain slices were obtained within 20 min. We succeeded in obtaining quantitative images of (1) [18F]fluorodeoxyglucose uptake, (2) dopamine D2 receptor binding, (3) dopa-decarboxylase activity, and (4) release of [11C]dopamine preloaded as L-[11C]DOPA in the brain slice preparation. These results demonstrate that positron emitter-labeled tracers in combination with storage phosphor screens are useful for functional imaging of living brain slices as a novel neuroscience technique.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Buffers,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyglucose,
http://linkedlifedata.com/resource/pubmed/chemical/Dopamine Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorodeoxyglucose F18,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Levodopa,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotransmitter Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Raclopride,
http://linkedlifedata.com/resource/pubmed/chemical/Salicylamides
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0168-0102
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
22
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
219-29
|
|
pubmed:dateRevised |
2005-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:7566703-Animals,
pubmed-meshheading:7566703-Brain,
pubmed-meshheading:7566703-Buffers,
pubmed-meshheading:7566703-Carbon Radioisotopes,
pubmed-meshheading:7566703-Culture Media,
pubmed-meshheading:7566703-Deoxyglucose,
pubmed-meshheading:7566703-Dopamine,
pubmed-meshheading:7566703-Dopamine Antagonists,
pubmed-meshheading:7566703-Fluorine Radioisotopes,
pubmed-meshheading:7566703-Fluorodeoxyglucose F18,
pubmed-meshheading:7566703-Glucose,
pubmed-meshheading:7566703-Kinetics,
pubmed-meshheading:7566703-Levodopa,
pubmed-meshheading:7566703-Male,
pubmed-meshheading:7566703-Neurotransmitter Agents,
pubmed-meshheading:7566703-Organ Culture Techniques,
pubmed-meshheading:7566703-Protein Binding,
pubmed-meshheading:7566703-Raclopride,
pubmed-meshheading:7566703-Rats,
pubmed-meshheading:7566703-Rats, Sprague-Dawley,
pubmed-meshheading:7566703-Salicylamides,
pubmed-meshheading:7566703-Tomography, Emission-Computed
|
|
pubmed:year |
1995
|
|
pubmed:articleTitle |
In vitro positron emission tomography (PET): use of positron emission tracers in functional imaging in living brain slices.
|
|
pubmed:affiliation |
Subfemtomole Biorecognition Project, Research Development Corporation of Japan, Osaka.
|
|
pubmed:publicationType |
Journal Article
|
