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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1995-10-25
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pubmed:abstractText |
Locus control regions (LCRs) are powerful assemblies of cis elements that organize the actions of cell-type-specific trans-acting factors. A 2.3-kb LCR in the human adenosine deaminase (ADA) gene first intron, which controls expression in thymocytes, is composed of a 200-bp enhancer domain and extended flanking sequences that facilitate activation from within chromatin. Prior analyses have demonstrated that the enhancer contains a 28-bp core region and local adjacent augmentative cis elements. We now show that the core contains a single critical c-Myb binding site. In both transiently cotransfected human cells and stable chromatin-integrated yeast cells, c-Myb strongly transactivated reporter constructs that contained polymerized core sequences. c-Myb protein was strongly evident in T lymphoblasts in which the enhancer was active and was localized within discrete nuclear structures. Fetal murine thymus exhibited a striking concordance of endogenous c-myb expression with that of mouse ADA and human ADA LCR-directed transgene expression. Point mutation of the c-Myb site within the intact 2.3-kb LCR severely attenuated enhancer activity in transfections and LCR activity in transgenic thymocytes. Within the context of a complex enhancer and LCR, c-Myb can act as an organizer of thymocyte-specific gene expression via a single binding site.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-1281545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-1340467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-1347906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-1508212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-1557416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-1709592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-1861984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-1944282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-2202948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-2405253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-2606352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-2748594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-3043180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-3511387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-6205276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-6392852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-6504046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-7525595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-7692896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-7823928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-7926766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-7954830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-7962048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-7969401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-8032204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-8152905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-8195231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-8195241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-8264615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-8293461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-8302594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-8321242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-8336720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-8406005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-8497265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565722-8510924
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5707-15
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7565722-Adenosine Deaminase,
pubmed-meshheading:7565722-Animals,
pubmed-meshheading:7565722-Base Sequence,
pubmed-meshheading:7565722-Binding Sites,
pubmed-meshheading:7565722-Cell Line,
pubmed-meshheading:7565722-Chromatin,
pubmed-meshheading:7565722-Enhancer Elements, Genetic,
pubmed-meshheading:7565722-Gene Expression Regulation, Developmental,
pubmed-meshheading:7565722-Humans,
pubmed-meshheading:7565722-Introns,
pubmed-meshheading:7565722-Mice,
pubmed-meshheading:7565722-Mice, Transgenic,
pubmed-meshheading:7565722-Molecular Sequence Data,
pubmed-meshheading:7565722-Point Mutation,
pubmed-meshheading:7565722-Proto-Oncogene Proteins,
pubmed-meshheading:7565722-Proto-Oncogene Proteins c-myb,
pubmed-meshheading:7565722-Saccharomyces cerevisiae,
pubmed-meshheading:7565722-T-Lymphocytes,
pubmed-meshheading:7565722-Thymus Gland,
pubmed-meshheading:7565722-Trans-Activators
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pubmed:year |
1995
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pubmed:articleTitle |
A central role for a single c-Myb binding site in a thymic locus control region.
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pubmed:affiliation |
Department of Pediatrics, Children's Hospital Medical Center, University of Cincinnati, Ohio 45229, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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