Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1995-10-25
pubmed:abstractText
The niaD and niiA genes of Aspergillus nidulans, which code, respectively, for nitrate and nitrite reductases, are divergently transcribed, and their ATGs are separated by 1,200 bp. The genes are under the control of the positively acting NirA transcription factor, which mediates nitrate induction. The DNA binding domain of NirA was expressed as a fusion protein with the glutathione S-transferase of Schistosoma japonicum. Gel shift and footprint experiments have shown that in the intergenic region there are four binding sites for the NirA transcription factor. These sites can be represented by the nonpalindromic consensus 5'CTCCGHGG3'. Making use of a bidirectional expression vector, we have analyzed the role of each of the sites in niaD and niiA expression. The sites were numbered from the niiA side. It appeared that site 1 is necessary for the inducibility of niiA only, while sites 2, 3, and to a lesser extent 4 (which is nearer to and strongly affects niaD) act bidirectionally. The results also suggest that of the 10 binding sites for the AreA protein, which mediates nitrogen metabolite repression, those which are centrally located are physiologically important. The insertion of an unrelated upstream activating sequence into the intergenic region strongly affected the expression of both genes, irrespective of the orientation in which the element was inserted.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-13040135, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1398125, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1400424, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1479914, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1538701, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1541396, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1557122, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1569930, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1595905, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1729616, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1756977, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1829047, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1829499, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1840634, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1916271, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1922075, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1970293, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1986367, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-1990284, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-2005880, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-2142530, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-2204810, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-2205530, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-2511324, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-2525625, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-265521, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-2668692, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-2670677, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-2689862, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-2830478, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-2951591, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-3043190, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-3323819, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-347305, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-3886158, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-389305, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-389740, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-4591376, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-5344606, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-5776531, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-6052720, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-6132393, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-6350263, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-6392852, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-7035292, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-7596294, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-7729421, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-7765125, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-7789806, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-8041355, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-8096840, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-8313886, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-8370541, http://linkedlifedata.com/resource/pubmed/commentcorrection/7565720-8437566
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:geneSymbol
niaD, niiA
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5688-99
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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