Linked Life Data

7561383

Source:http://linkedlifedata.com/resource/pubmed/id/7561383

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-11-22
pubmed:abstractText
CHO K1-cells, arrested in G0/G1 phase were irradiated with graded doses of 150-kV X-rays, and the dicentric chromosome aberrations in the first metaphase after reincubation in full growth medium were evaluated. When 500 microM ara A was administered at least 1 h before irradiation, maximal enhancement of the dicentric yield was obtained under 'immediate plating' conditions as well as under 'delayed plating' conditions, and under both conditions only the linear component alpha D of the aberration yield was affected. Since ara A had no effect upon the number of induced dsbs, as measured with PFGE at doses up to 100 Gy, the action pathway of ara A is suggested to be the repair/misrepair process manifested in the linear component of the dicentric yield. By varying the time at which either ara A at 37 degrees C was administered before irradiation or ara A at 4 degrees C was added after irradiation, it was observed that the repair step sensitive to ara A was completed within about 5 min after irradiation. This is in agreement with other observations of ara A action upon a fast repair step. The conversion of DNA single-strand breaks or base damage sites into double-strand breaks as a consequence of polymerase beta inhibition by ara A would explain the present results.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0955-3002
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
235-43
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Influence of ara A on the formation of dicentrics in irradiated quiescent CHO cells.
pubmed:affiliation
Institut für Medizinische Physik und Biophysik, Universität, Göttingen, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't