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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1995-11-13
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pubmed:databankReference |
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pubmed:abstractText |
Anti-Mi-2 autoantibody is strongly associated with dermatomyositis and found in sera of 20% of patients. Mi-2 antigen contains at least eight components and previous evidence suggested that the 240-kD protein was the antigenic component for at least some sera. In this study, anti-M-2 patient sera were used to screen human thymocyte and HeLa cell lambda gt11 expression libraries, and two clones from each had plaques specifically reactive with anti-Mi-2 sera. Studies with affinity-purified antibody supported the identification of the clones. All of 44 anti-Mi-2 sera reacted with the plaques, but none of 44 control sera reacted significantly. The cDNAs were identical, and full sequencing of one revealed an open reading frame spanning a 1,054-bp insert. Rescreening the library with the cDNA yielded a 1,589-bp cDNA that continued the open reading frame. The Mi-2 cDNA hybridized to a single 7.5-8.0 kb mRNA of HeLa cells, by Northern blot. Rabbit antiserum directed at a portion of the cDNA product reacted with HeLa 240-kD Mi-2 protein. The sequence was notable for four potential zinc-fingers and several charged regions. The protein encoded by the cDNA produced in vitro reacted with only one of five of the Mi-2 sera. These findings indicate that the Mi-2 240 kD is a novel protein that is antigenic for all Mi-2 sera, and strongly suggests that a major common epitope is conformational in nature.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-1378227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-1549558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-1644924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-1659647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-1996354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-2231712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-2307838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-2403400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-2409985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-2421409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-2524838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-2526016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-2876518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-3028647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-3260384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-3273187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-3275869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-3313277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-3319186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-3367995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-3701255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-6206177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-6219389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-6866113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-7406938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-7818561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-7945480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-8358434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-8423380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7560064-8514867
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
96
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1730-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7560064-Adenosine Triphosphatases,
pubmed-meshheading:7560064-Amino Acid Sequence,
pubmed-meshheading:7560064-Animals,
pubmed-meshheading:7560064-Autoantibodies,
pubmed-meshheading:7560064-Autoantigens,
pubmed-meshheading:7560064-Base Sequence,
pubmed-meshheading:7560064-DNA, Complementary,
pubmed-meshheading:7560064-DNA Helicases,
pubmed-meshheading:7560064-Dermatomyositis,
pubmed-meshheading:7560064-Epitopes,
pubmed-meshheading:7560064-HeLa Cells,
pubmed-meshheading:7560064-Humans,
pubmed-meshheading:7560064-Male,
pubmed-meshheading:7560064-Mi-2 Nucleosome Remodeling and Deacetylase Complex,
pubmed-meshheading:7560064-Molecular Sequence Data,
pubmed-meshheading:7560064-Molecular Weight,
pubmed-meshheading:7560064-Rabbits,
pubmed-meshheading:7560064-Zinc Fingers
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pubmed:year |
1995
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pubmed:articleTitle |
Molecular analysis of a major antigenic region of the 240-kD protein of Mi-2 autoantigen.
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pubmed:affiliation |
Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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