Linked Life Data

7559890

Source:http://linkedlifedata.com/resource/pubmed/id/7559890

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1995-11-7
pubmed:abstractText
In continuation of earlier observations on the involvement of interleukin-1 (IL-1) in ovarian function, we examined the ability of IL-1 to modulate plasminogen activator (PA) activity and prostaglandin (PG) synthesis in human granulosa lutein cells (GLCs). Toward this goal, GLCs were obtained from women undergoing in vitro fertilization, preincubated with 10% fetal calf serum for 48 h, and subsequently cultured for 48 h in serum-free media in the absence or presence of IL-1 beta (10 ng/mL). Cellular PA activity was measured by plasminogen-dependent cleavage of the chromogenic substrate H-D-valyl-L-leucyl-L-lysine-p-nitroanilide (S-2251). Prostaglandin E (PGE) levels were assayed by conventional RIA. Exposure of GLCs to IL-1 resulted in a 50% increase in PGE production, a 33% suppression of PA activity, and a 75% increase in the ability of the corresponding conditioned media to inhibit exogenous urokinase activity. The inhibitory capacity was attributable to an IL-1-mediated increase in PA inhibitor type-1 (PAI-1) production, inasmuch as urokinase inhibition could be abolished by the administration of a polyclonal antihuman PAI-1 immunoglobulin G. IL-1 treatment had no effect on plasmin or trypsin inhibition. Exposure of GLCs to IL-1 receptor antagonist abolished the ability of IL-1 to enhance PA inhibitory activity and PGE production, thereby establishing specific IL-1 receptor-mediated effects. The ability of IL-1 to suppress PA activity and to produce PAI-1 persisted in the presence of indomethacin, a potent inhibitor of PG synthesis. Likewise, transforming growth factor-beta 1 suppressed the ability of IL-1 to stimulate PGE production without affecting the IL-1-induced effects on the PA system. The present findings suggest a pluripotent response of GLCs to IL-1, characterized by the induction of PAI-1 and the suppression of PA occurring concurrent with, but independent of, PG production. These observations support the potential involvement of IL-1 in the regulation of human ovulatory processes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-972X
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3018-24
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:7559890-Amino Acid Sequence, pubmed-meshheading:7559890-Cells, Cultured, pubmed-meshheading:7559890-Corpus Luteum, pubmed-meshheading:7559890-Culture Media, Serum-Free, pubmed-meshheading:7559890-Female, pubmed-meshheading:7559890-Fertilization in Vitro, pubmed-meshheading:7559890-Granulosa Cells, pubmed-meshheading:7559890-Humans, pubmed-meshheading:7559890-Interleukin 1 Receptor Antagonist Protein, pubmed-meshheading:7559890-Interleukin-1, pubmed-meshheading:7559890-Molecular Sequence Data, pubmed-meshheading:7559890-Ovulation, pubmed-meshheading:7559890-Plasminogen Activator Inhibitor 1, pubmed-meshheading:7559890-Plasminogen Activators, pubmed-meshheading:7559890-Prostaglandins E, pubmed-meshheading:7559890-Radioimmunoassay, pubmed-meshheading:7559890-Sialoglycoproteins, pubmed-meshheading:7559890-Substrate Specificity, pubmed-meshheading:7559890-Urokinase-Type Plasminogen Activator
pubmed:year
1995
pubmed:articleTitle
Interleukin-1-mediated stimulation of prostaglandin E production is without effect on plasminogen activator activity in human granulosa lutein cell cultures.
pubmed:affiliation
Department of Obstetrics/Gynecology, Hadassah University Hospital, Mt. Scopus, Jerusalem, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't